Sharples G J, Benson F E, Illing G T, Lloyd R G
Genetics Department, University of Nottingham, Queens Medical Centre, UK.
Mol Gen Genet. 1990 Apr;221(2):219-26. doi: 10.1007/BF00261724.
Recombinant plasmids carrying ruvA, ruvB, or both were constructed and used to investigate the genetic defects in a collection of UV-sensitive ruv mutants. The results revealed that efficient survival of UV-irradiated cells depends on both ruvA and ruvB, and on a third gene, ruvC, located upstream of the ruvAB operon. Southern blotting analysis was used to locate insertions in ruv and to examine putative deletion mutants. Two Tn10 insertions were located to the region encoding ruvA. Since these insertions caused a deficiency in the activities of both ruvA and ruvB, we concluded that they must exert a polar effect on ruvB. Two putative ruv deletion mutants were shown to be the result of deletion-inversion events mediated during imprecise excision of Tn10. The relevant inversion breakpoints in these mutants were located to ruvA and ruvC.
构建了携带ruvA、ruvB或两者的重组质粒，并用于研究一组对紫外线敏感的ruv突变体中的遗传缺陷。结果表明，紫外线照射细胞的有效存活取决于ruvA和ruvB，以及位于ruvAB操纵子上游的第三个基因ruvC。Southern印迹分析用于定位ruv中的插入并检查推定的缺失突变体。两个Tn10插入位于编码ruvA的区域。由于这些插入导致ruvA和ruvB的活性均缺乏，我们得出结论，它们必定对ruvB产生极性效应。两个推定的ruv缺失突变体被证明是Tn10不精确切除过程中介导的缺失-倒位事件的结果。这些突变体中的相关倒位断点位于ruvA和ruvC。
