Ng G Y, Oakes B W, Deacon O W, McLean I D, Eyre D R
Department of Rehabilitation Sciences, Hong Kong Polytechnic University, Hung Hom, Kowloon.
J Orthop Res. 1996 Nov;14(6):851-6. doi: 10.1002/jor.1100140602.
This study examined the change in type-III collagen concentration and hydroxypyridinium crosslink density of anterior cruciate ligament-patellar tendon autografts and their correlations with Young's modulus of the anterior cruciate autografts and anterior cruciate controls for as long as 3 years after surgery. Fifteen adult female goats (two control and 13 experimental) were tested. Each experimental animal received an anterior cruciate ligament-patellar tendon autograft to the right knee. These animals were tested at 0 (n = 2), 6 (n = 2), 12 (n = 2), and 24 (n = 1) weeks and 1 (n = 3) and 3 (n = 3) years after surgery. After mechanical testing, the anterior cruciate autograft and control tissues were analysed for type-III collagen concentration and hydroxypyridinium crosslink density. The results of sodium dodecyl sulfate gel electrophoresis showed a trend of initial increase in the percentage of type-III collagen in the anterior cruciate ligament autografts and a subsequent decrease after 24 weeks following surgery. There was a nonsignificant (p > 0.05) negative correlation between type-III collagen concentration and Young's modulus. The hydroxypyridinium crosslink density was highest at 1 year after surgery. There was a significant (p < 0.05) positive correlation between hydroxypyridinium crosslink density and Young's modulus in the anterior cruciate autografts and controls. This suggests that hydroxypyridinium crosslink density has a good linear relationship with the material strength of the anterior cruciate ligament autograft and hence could be used as an objective guide for rehabilitation with anterior cruciate autografts.
本研究检测了前交叉韧带-髌腱自体移植物中III型胶原浓度和羟基吡啶交联密度的变化，以及它们与前交叉韧带自体移植物和前交叉韧带对照物的杨氏模量之间的相关性，随访时间长达术后3年。对15只成年雌性山羊（2只为对照，13只为实验动物）进行了检测。每只实验动物的右膝均接受了前交叉韧带-髌腱自体移植。在术后0周（n = 2）、6周（n = 2）、12周（n = 2）、24周（n = 1）以及1年（n = 3）和3年（n = 3）对这些动物进行检测。在力学测试后，分析前交叉韧带自体移植物和对照组织的III型胶原浓度和羟基吡啶交联密度。十二烷基硫酸钠凝胶电泳结果显示，前交叉韧带自体移植物中III型胶原百分比最初呈上升趋势，术后24周后随后下降。III型胶原浓度与杨氏模量之间存在无统计学意义（p > 0.05）的负相关。羟基吡啶交联密度在术后1年时最高。在前交叉韧带自体移植物和对照物中，羟基吡啶交联密度与杨氏模量之间存在显著（p < 0.05）的正相关。这表明羟基吡啶交联密度与前交叉韧带自体移植物的材料强度具有良好的线性关系，因此可作为前交叉韧带自体移植物康复的客观指导。
