Hsu S C, Ting A E, Hazuka C D, Davanger S, Kenny J W, Kee Y, Scheller R H
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical School, California 94305-5428, USA.
Neuron. 1996 Dec;17(6):1209-19. doi: 10.1016/s0896-6273(00)80251-2.
rsec6 and rsec8 are two components of a 17S complex in mammalian brain that is homologous to the yeast 834 kDa Sec6/8/15 complex which is essential for exocytosis. Purification and partial amino acid sequencing of the mammalian rsec6/8 complex reveals that it is composed of eight novel proteins with a combined molecular weight of 743 kDa. The complex is broadly expressed in brain and displays a plasma membrane localization in nerve terminals. Membrane associated rsec6/8 complex coimmunoprecipitates with syntaxin, a plasma membrane protein critical for neurotransmission. These data suggest a role for the mammalian rsec6/8 complex in neurotransmitter release via interactions with the core vesicle docking and fusion apparatus.
Rsec6和Rsec8是哺乳动物大脑中17S复合物的两个组成部分，该复合物与酵母834 kDa Sec6/8/15复合物同源，后者对胞吐作用至关重要。哺乳动物rsec6/8复合物的纯化和部分氨基酸测序表明，它由8种新蛋白质组成，总分子量为743 kDa。该复合物在大脑中广泛表达，并在神经末梢的质膜定位。与膜相关的rsec6/8复合物与 syntaxin（一种对神经传递至关重要的质膜蛋白）进行共免疫沉淀。这些数据表明，哺乳动物rsec6/8复合物通过与核心囊泡对接和融合装置相互作用，在神经递质释放中发挥作用。
