Quilliam M A, Templeton J F, Westmore J B
Steroids. 1977 May;29(5):613-26. doi: 10.1016/0039-128x(77)90013-7.
The applications of sterically crowed trialkyksilyl ether derivatives to the analysis and characterization by thin-layer chromatography, gas chromatography and mass spectrometry, of metabolites of 2alpha, 3alpha-cyclopropano-5alpha-androstan-17beta-ol in the rabbit are described. These derivatives are complementary to the familiar trimethysilyl ether derivatives, but have greater hydrolytic stability (an advantage for TLC), generally give better GC separations, and have characteristic mass spectra. Isomer differentiation by GC and MS is also more readily achieved than via the TMSi ether derivatives. These properties should make SCTASi ethers useful derivatives for studies of steroid metabolism.
描述了空间拥挤的三烷基甲硅烷基醚衍生物在兔体内2α,3α - 环丙烷 - 5α - 雄甾烷 - 17β - 醇代谢产物的薄层色谱、气相色谱和质谱分析与表征中的应用。这些衍生物是常见的三甲基甲硅烷基醚衍生物的补充，但具有更高的水解稳定性（这对薄层色谱是一个优势），通常能实现更好的气相色谱分离，并且具有特征性质谱。与通过三甲基硅醚衍生物相比，通过气相色谱和质谱进行异构体区分也更容易实现。这些特性应使三烷基甲硅烷基醚成为甾体代谢研究中有用的衍生物。
