Templeton J F, Kim R S
Steroids. 1975 Mar;25(3):403-11. doi: 10.1016/0039-128x(75)90096-3.
Metabolism of 5alpha-androstan-3-one in the rabbit was studied in order to investigate changes in the steroidal ring system. The total crude urinary extract was separated into a non-ketonic and a ketonic fraction and was further purified by column chromatography. 3-alpha- and 3-beta-Hydroxy-5-alpha-androstan-16-one together with 5-alpha-androstane-3-alpha, 16-alpha- and 3-beta, 16-alpha-diol, and 5-alpha-androstane-3-beta, 17-alpha-diol have been isolated and identified by spectroscopic methods and comparison with authentic samples. The results were compared with other metabolic studies of steroid derivatives. From this study it is suggested that metabolic oxidation occurs in positions removed from the initial oxygen function of the monooxygenated steroids.
为了研究甾体环系统的变化，对家兔体内5α-雄甾烷-3-酮的代谢进行了研究。将总尿粗提物分离为非酮部分和酮部分，并通过柱色谱进一步纯化。通过光谱方法并与标准样品比较，分离并鉴定出了3-α-和3-β-羟基-5-α-雄甾烷-16-酮以及5-α-雄甾烷-3-α,16-α-二醇、3-β,16-α-二醇和5-α-雄甾烷-3-β,17-α-二醇。将结果与甾体衍生物的其他代谢研究进行了比较。从这项研究可以看出，代谢氧化发生在单加氧甾体初始氧官能团以外的位置。
