Nakae H, Endo S, Inada K, Yamada Y, Takakuwa T, Yoshida M
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Burns. 1996 Dec;22(8):594-7. doi: 10.1016/s0305-4179(96)00063-0.
Plasma concentrations of endothelin-1 (ET-1) and thrombomodulin (TM) were determined in patients with burns to examine their relation to the severity of illness. Tumor necrosis factor-alpha (TNF-alpha) was also measured, and its relationship to ET-1 and TM determined. Twenty-three burn patients were evaluated, who had a total burn surface area (TBSA) of at least 20 per cent. ET-1 was measured by radioimmunoassay (RIA). TM and TNF-alpha were measured by enzyme-linked immunosorbent assay (ELISA). Both the ET-1 and TM concentrations were significantly higher in the patients who developed sepsis than in those who did not and in the patients who eventually died than in those who survived. Maximum plasma concentrations of ET-1 and TM were significantly correlated with the acute physiological and chronic health evaluation II score. There was also a significant correlation between the plasma levels of TNF-alpha and both ET-1 and TM. ET-1 and TM closely reflect the severity of illness in patients with burns in the infectious stage; TNF-alpha may be involved in the production of ET-1 and TM.
测定烧伤患者血浆中内皮素-1（ET-1）和血栓调节蛋白（TM）的浓度，以研究它们与疾病严重程度的关系。还检测了肿瘤坏死因子-α（TNF-α），并确定其与ET-1和TM的关系。对23例烧伤患者进行了评估，这些患者的烧伤总面积（TBSA）至少为20%。采用放射免疫分析法（RIA）测定ET-1。采用酶联免疫吸附测定法（ELISA）测定TM和TNF-α。发生脓毒症的患者ET-1和TM浓度均显著高于未发生脓毒症的患者，最终死亡的患者ET-1和TM浓度均显著高于存活患者。ET-1和TM的血浆最大浓度与急性生理和慢性健康状况评估II评分显著相关。TNF-α的血浆水平与ET-1和TM也显著相关。ET-1和TM密切反映了感染期烧伤患者的疾病严重程度；TNF-α可能参与了ET-1和TM的产生。
