Elankumaran S, Raj G D, Thangavelu A, Koteeswaran A, Albert A, Venugopalan A T
Centre For Animal Health Studies, Tamilnadu Veterinary and Animal Sciences University, Madras, India.
Trop Anim Health Prod. 1996 Nov;28(4):293-7. doi: 10.1007/BF02240821.
Micro-haemagglutination inhibition tests (Micro-HI) were used to measure the level of maternal IgG in the tears of chicks and also to measure the levels of HI antibodies in the tears and serum after vaccination with "F" strain of Newcastle disease virus (NDV) and in the face of an outbreak of Newcastle disease. There was a 1.4 fold difference between the maternal IgG concentration in the serum and tears. The ratio of serum IgG to lachrymal IgG after maternal transfer was 4 to 5:1 on day 4 to 9 and decreased to 2.6:1 on day 12 post-hatch. The intra-ocular vaccination of chicks with "F" strain of NDV resulted in the highest titre of HI antibodies in the tears though there was no significant difference in the response of chicks vaccinated through intranasal, oral and intravenous routes. In the face of an ND outbreak, the level of HI antibodies in the tears during the acute phase was very high and persisted at the same level for 14 days.
采用微量血凝抑制试验（Micro-HI）来检测雏鸡泪液中母源IgG水平，同时检测用新城疫病毒（NDV）“F”株疫苗接种后以及在新城疫爆发时雏鸡泪液和血清中的血凝抑制（HI）抗体水平。血清和泪液中母源IgG浓度存在1.4倍的差异。母源抗体转移后，第4至9天血清IgG与泪液IgG的比例为4至5:1，出壳后第12天降至2.6:1。用NDV“F”株对雏鸡进行眼内接种，泪液中HI抗体滴度最高，不过通过鼻内、口服和静脉途径接种的雏鸡在反应上没有显著差异。在新城疫爆发时，急性期泪液中的HI抗体水平非常高，并在14天内维持在同一水平。
