Roy P, Koteeswaran A, Sridevi P, Venugopalan A T
Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madras, India.
Trop Anim Health Prod. 1998 Feb;30(1):31-5. doi: 10.1023/a:1005009309497.
Seroconversion of 3 lentogenic commercial Newcastle disease (ND) vaccines and experimental V4 vaccines was compared based on the haemagglutination inhibition (HI) test against ND. It was found that for primary vaccination all the vaccines produced similar response but for vaccinations V4 and LaSota were better than RDVF. Eight-five samples each of serum, tears and feather pulp were collected from respective birds and antibody assessment was done against ND by HI test. The geometric mean HI titres (GMT) of serum samples were highest followed by tears and feather pulp samples before vaccination and 3 weeks after vaccination by oculonasal route and the difference was statistically significant (p < 0.01). Three weeks after booster vaccination by oculonasal route, however, the GMT of serum samples were highest followed by feather pulp and tear samples. The ease of collection of feather pulp samples and their role in ND serology is discussed.
基于针对新城疫的血凝抑制（HI）试验，比较了3种弱毒商业新城疫（ND）疫苗和实验性V4疫苗的血清转化情况。结果发现，对于初次接种，所有疫苗产生的反应相似，但对于再次接种，V4和LaSota疫苗比拉杜立克斯疫苗（RDVF）更好。从各自的鸟类中分别采集了85份血清、眼泪和羽毛髓样本，并通过HI试验针对新城疫进行了抗体评估。接种疫苗前以及经眼鼻途径接种疫苗3周后，血清样本的几何平均血凝抑制滴度（GMT）最高，其次是眼泪和羽毛髓样本，差异具有统计学意义（p < 0.01）。然而，经眼鼻途径加强免疫3周后，血清样本的GMT最高，其次是羽毛髓和眼泪样本。讨论了羽毛髓样本采集的便利性及其在新城疫血清学中的作用。
