Surchev L
Mol Med (Sofia). 1996;1(2):28-34.
In the present review, both subkinds of the method for membrane splitting, namely freeze-etching and freeze-fracturing techniques, are presented. After a brief historical survey, a mechanism of their action is described. Special attention is paid to the consecutive stages in applying these techniques--fixation, fracturing, etching, replication, cleaning and observation. A new idea for a better cleaning of the replicas and an explanation of the different occurrence of intramembranous particles on the membrane fracture faces are proposed. Finally, the application of the freeze-etching and freeze-fracturing techniques to visualize the intramembranous structure of membranes in the central nervous system is outlined.
在本综述中，介绍了膜分裂方法的两种类型，即冷冻蚀刻和冷冻断裂技术。在简短的历史回顾之后，描述了它们的作用机制。特别关注了应用这些技术的连续阶段——固定、断裂、蚀刻、复制、清洗和观察。提出了一种更好地清洗复制品的新想法，并解释了膜断裂面上膜内颗粒不同出现情况的原因。最后，概述了冷冻蚀刻和冷冻断裂技术在可视化中枢神经系统膜内结构方面的应用。
