Angelopoulou K, Stratis M, Diamandis E P
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Canada.
Int J Cancer. 1997 Jan 6;70(1):46-51. doi: 10.1002/(sici)1097-0215(19970106)70:1<46::aid-ijc7>3.0.co;2-6.
p53 aberrations are frequent in colorectal carcinogenesis (40-70%). Because p53 gene mutations typically result in increased p53 protein concentration in tumor cells, this cellular protein might become immunogenic during tumor development. To test this hypothesis, serum p53 antibodies were quantitatively analyzed in 229 patients with colorectal cancer, using an immunofluorometric procedure. Circulating antibodies against p53 were found in 23% (53/229) of the patients. We quantified antibody concentrations in all positive sera and found that they varied from 300 to 500,000 arbitrary units/1. Sequential analysis of positive sera from 3 patients showed that p53 antibody concentrations change during the course of the disease, reflecting progression or regression. No association was found between the presence of p53 antibodies and age, sex, stage, histological grade and patient relapse-free or overall survival. These data demonstrate that antibody generation against the p53 tumor-suppressor protein is a relatively common event in colorectal cancer and that serological analysis for p53 antibodies may have some value for patient monitoring. The test has no value for prognosis.
p53畸变在结直肠癌发生过程中很常见（40%-70%）。由于p53基因突变通常会导致肿瘤细胞中p53蛋白浓度升高，这种细胞蛋白在肿瘤发展过程中可能会变得具有免疫原性。为了验证这一假设，采用免疫荧光测定法对229例结直肠癌患者的血清p53抗体进行了定量分析。在23%（53/229）的患者中发现了循环抗p53抗体。我们对所有阳性血清中的抗体浓度进行了定量，发现其范围为300至500,000任意单位/升。对3例患者的阳性血清进行的连续分析表明，p53抗体浓度在疾病过程中会发生变化，反映疾病的进展或缓解。未发现p53抗体的存在与年龄、性别、分期、组织学分级以及患者无复发生存期或总生存期之间存在关联。这些数据表明，针对p53肿瘤抑制蛋白产生抗体在结直肠癌中是相对常见的事件，并且对p53抗体进行血清学分析可能对患者监测具有一定价值。该检测对预后没有价值。
