Henke W, Jung M, Jung K, Lein M, Schlechte H, Berndt C, Rudolph B, Schnorr D, Loening S A
Klinik und Poliklinik für Urologie, Universitätsklinikum Charité, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.
Int J Cancer. 1997 Jan 6;70(1):52-6. doi: 10.1002/(sici)1097-0215(19970106)70:1<52::aid-ijc8>3.0.co;2-5.
The diagnostic specificity of the detection of disseminated prostatic cells by reverse-transcriptase polymerase chain reaction (RT-PCR) of PSA mRNA was investigated. A sensitive nested PCR was developed. In blood samples from 10 healthy female and 10 healthy male persons examined by RT-PCR, mRNA of PSA was detected 3 times in each group. In the groups of patients suffering from benign prostate hyperplasia and prostate cancer, 6 of 11 and 5 of 12, respectively, gave positive RT-PCR results. With increasing analytical sensitivity of the RT-PCR of PSA mRNA, the diagnostic specificity of the assay is decreased. Further development of this diagnostic method requires the introduction of the quantitative PCR which may make possible discrimination between prostatic and non-prostatic source of PSA mRNA by quantification.
通过前列腺特异性抗原（PSA）mRNA的逆转录聚合酶链反应（RT-PCR）检测播散性前列腺细胞的诊断特异性进行了研究。开发了一种灵敏的巢式PCR。在通过RT-PCR检测的10名健康女性和10名健康男性的血液样本中，每组均检测到3次PSA的mRNA。在良性前列腺增生和前列腺癌患者组中，分别有11例中的6例和12例中的5例RT-PCR结果呈阳性。随着PSA mRNA的RT-PCR分析灵敏度的提高，该检测方法的诊断特异性降低。这种诊断方法的进一步发展需要引入定量PCR，这可能通过定量来区分PSA mRNA的前列腺来源和非前列腺来源。
