Boursnell M E, Entwisle C, Blakeley D, Roberts C, Duncan I A, Chisholm S E, Martin G M, Jennings R, Ni Challanaín D, Sobek I, Inglis S C, McLean C S
Cantab Pharmaceuticals Research Ltd., Cambridge, United Kingdom.
J Infect Dis. 1997 Jan;175(1):16-25. doi: 10.1093/infdis/175.1.16.
A glycoprotein H (gH)-deleted herpes simplex virus type 2 (HSV-2) was evaluated as a vaccine for the prevention of HSV-induced disease. This virus, which we term a DISC (disabled infectious single cycle) virus, can only complete one replication cycle in normal cells and should thus be safe yet still able to stimulate broad humoral and cell-mediated antiviral immune responses. A gH-deleted HSV-2 virus that has been tested as a vaccine in the guinea pig model of recurrent HSV-2 infection was constructed. Animals vaccinated with DISC HSV-2 showed complete protection against primary HSV-2-induced disease, even when challenged 6 months after vaccination. In addition, the animals were almost completely protected against recurrent disease. Even at low vaccination doses, there was a high degree of protection against primary disease. A reduction in recurrent disease symptoms was also observed following therapeutic vaccination of animals already infected with wild type HSV-2.
一种缺失糖蛋白H（gH）的2型单纯疱疹病毒（HSV - 2）被评估作为预防HSV引起疾病的疫苗。这种病毒，我们称之为DISC（失活感染单周期）病毒，在正常细胞中只能完成一个复制周期，因此应该是安全的，但仍能够刺激广泛的体液和细胞介导的抗病毒免疫反应。构建了一种已在复发性HSV - 2感染的豚鼠模型中作为疫苗进行测试的缺失gH的HSV - 2病毒。接种DISC HSV - 2的动物对原发性HSV - 2引起的疾病表现出完全的保护作用，即使在接种后6个月受到攻击时也是如此。此外，这些动物几乎完全受到保护，免受复发性疾病的侵害。即使在低疫苗接种剂量下，对原发性疾病也有高度的保护作用。在对已经感染野生型HSV - 2的动物进行治疗性疫苗接种后，也观察到复发性疾病症状有所减轻。
