McLean C S, Erturk M, Jennings R, Challanain D N, Minson A C, Duncan I, Boursnell M E, Inglis S C
Cantab Pharmaceuticals Research Ltd., Cambridge, United Kingdom.
J Infect Dis. 1994 Nov;170(5):1100-9. doi: 10.1093/infdis/170.5.1100.
The vaccine potential of a mutant herpes simplex virus (HSV) type 1, with a deletion in the glycoprotein H (gH) gene, was evaluated. The virus requires a gH-expressing cell line for multi-cycle growth but can complete a single cycle of infection in noncomplementing cells. Such viruses, termed DISC (disabled infectious single cycle) viruses, should be safe, yet still able to stimulate humoral and cell-mediated responses against a broad range of virus antigens in vaccinated hosts. Prophylactic vaccination of guinea pigs with DISC HSV-1, by ear scarification or direct infection of the vaginal mucosa, afforded a high degree of protection against HSV-2-induced primary genital disease and reduced significantly the frequency of subsequent disease recurrence. There was also a trend toward reduced recurrence following therapeutic vaccination of animals already infected with HSV-2. DISC HSV vaccination, therefore, offers an effective route for control of HSV disease.
对一种1型单纯疱疹病毒（HSV）突变体的疫苗潜力进行了评估，该突变体的糖蛋白H（gH）基因存在缺失。该病毒多轮生长需要一个表达gH的细胞系，但能在非互补细胞中完成单个感染周期。这类病毒被称为DISC（缺陷感染性单周期）病毒，应该是安全的，并且仍能够在接种疫苗的宿主中刺激针对多种病毒抗原的体液免疫和细胞介导免疫反应。通过耳部划痕或直接感染阴道黏膜，用DISC HSV-1对豚鼠进行预防性接种，可提供高度保护，防止HSV-2诱导的原发性生殖器疾病，并显著降低后续疾病复发的频率。对于已经感染HSV-2的动物进行治疗性接种后，复发频率也有降低的趋势。因此，DISC HSV疫苗接种为控制HSV疾病提供了一条有效途径。
