Busse J, Hartmann E, Lingwood C A
Department of Microbiology, Research Institute, Hospital for Sick Children, Toronto, Canada.
J Infect Dis. 1997 Jan;175(1):77-83. doi: 10.1093/infdis/175.1.77.
Thirteen clinical strains of Haemophilus influenzae, including types b, d, and untypeable, in vitro specifically recognize phosphatidylethanolamine (PE), gangliotetraosylceramide, gangliotriosylceramide (Gg3), sulfatoxygalactosylceramide, and to a lesser extent sulfatoxygalactosylglycerol. A PE affinity matrix was used to purify an adhesin of approximately 46 kDa from both type b and untypeable H. influenzae. This adhesin was a potent inhibitor of H. influenzae Gg3 and PE binding in vitro, and polyclonal antibodies specific for this protein prevented the attachment of H. influenzae Gg3 and PE and cultured HEp-2 epithelial cells in vitro.
13株流感嗜血杆菌临床菌株，包括b型、d型和不可分型，在体外能特异性识别磷脂酰乙醇胺（PE）、神经节四糖神经酰胺、神经节三糖神经酰胺（Gg3）、硫酸化半乳糖基神经酰胺，以及程度较轻的硫酸化半乳糖基甘油。使用PE亲和基质从b型和不可分型流感嗜血杆菌中纯化出一种约46 kDa的黏附素。这种黏附素在体外是流感嗜血杆菌Gg3和PE结合的有效抑制剂，针对该蛋白的多克隆抗体在体外可阻止流感嗜血杆菌Gg3和PE与培养的HEp-2上皮细胞的附着。
