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热诱导表面应激蛋白（Hsp70）介导呼吸道病原体流感嗜血杆菌对硫脂的识别。

Heat-inducible surface stress protein (Hsp70) mediates sulfatide recognition of the respiratory pathogen Haemophilus influenzae.

作者信息

Hartmann E, Lingwood C A, Reidl J

机构信息

Zentrum für Infektionsforschung, Universität Würzburg, Röntgenring 11, Würzburg, Germany.

出版信息

Infect Immun. 2001 May;69(5):3438-41. doi: 10.1128/IAI.69.5.3438-3441.2001.

DOI:10.1128/IAI.69.5.3438-3441.2001
PMID:11292771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC98307/
Abstract

The in vitro glycolipid binding specificity of clinical strains of nontypeable Haemophilus influenzae is altered to include sulfated glycolipids following a brief heat shock. We have constructed, expressed, and purified a recombinant protein of H. influenzae Hsp70, which showed significant specific binding to sulfated galactolipids in vitro. Furthermore, indirect immunofluorescence demonstrates that Hsp70 proteins are surface exposed in H. influenzae only after heat shock and are contained in the outer membrane protein fractions.

摘要

不可分型流感嗜血杆菌临床菌株的体外糖脂结合特异性在短暂热休克后发生改变，包括与硫酸化糖脂结合。我们构建、表达并纯化了流感嗜血杆菌Hsp70的重组蛋白，该蛋白在体外显示出与硫酸化半乳糖脂有显著的特异性结合。此外，间接免疫荧光显示，Hsp70蛋白仅在热休克后暴露于流感嗜血杆菌表面，并存在于外膜蛋白组分中。

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Heat-inducible surface stress protein (Hsp70) mediates sulfatide recognition of the respiratory pathogen Haemophilus influenzae.热诱导表面应激蛋白（Hsp70）介导呼吸道病原体流感嗜血杆菌对硫脂的识别。
Infect Immun. 2001 May;69(5):3438-41. doi: 10.1128/IAI.69.5.3438-3441.2001.
2
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本文引用的文献

1
Hsp70s contain a specific sulfogalactolipid binding site. Differential aglycone influence on sulfogalactosyl ceramide binding by recombinant prokaryotic and eukaryotic hsp70 family members.热休克蛋白70（Hsp70s）含有一个特定的硫代半乳糖脂结合位点。不同苷元对重组原核和真核热休克蛋白70家族成员结合硫代半乳糖基神经酰胺的影响。
Biochemistry. 2001 Mar 27;40(12):3572-82. doi: 10.1021/bi001643u.
2
The ATPase domain of hsp70 possesses a unique binding specificity for 3'-sulfogalactolipids.热休克蛋白70（hsp70）的ATP酶结构域对3'-磺基半乳糖脂具有独特的结合特异性。
J Biol Chem. 2001 Jan 5;276(1):449-56. doi: 10.1074/jbc.M006732200.
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NADP and NAD utilization in Haemophilus influenzae.流感嗜血杆菌中烟酰胺腺嘌呤二核苷酸磷酸（NADP）和烟酰胺腺嘌呤二核苷酸（NAD）的利用情况
Mol Microbiol. 2000 Mar;35(6):1573-81. doi: 10.1046/j.1365-2958.2000.01829.x.
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The Hsp70 and Hsp60 chaperone machines.热休克蛋白70（Hsp70）和热休克蛋白60（Hsp60）伴侣机制。
Cell. 1998 Feb 6;92(3):351-66. doi: 10.1016/s0092-8674(00)80928-9.
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Expression and sulfogalactolipid binding specificity of the recombinant testis-specific cognate heat shock protein 70.重组睾丸特异性同源热休克蛋白70的表达及硫代半乳糖脂结合特异性
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J Immunol. 1997 May 1;158(9):4341-50.
7
Brief heat shock treatment induces a long-lasting alteration in the glycolipid receptor binding specificity and growth rate of Haemophilus influenzae.短暂热休克处理会导致流感嗜血杆菌的糖脂受体结合特异性和生长速率发生持久改变。
Infect Immun. 1997 May;65(5):1729-33. doi: 10.1128/iai.65.5.1729-1733.1997.
8
Receptor affinity purification of a lipid-binding adhesin from Haemophilus influenzae.从流感嗜血杆菌中通过受体亲和纯化脂质结合黏附素
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Infect Immun. 1996 Mar;64(3):905-12. doi: 10.1128/iai.64.3.905-912.1996.