Miyazaki S, Nunoya T, Matsumoto T, Tateda K, Yamaguchi K
Department of Microbiology, School of Medicine, Toho University, Tokyo, Japan.
J Infect Dis. 1997 Jan;175(1):205-9. doi: 10.1093/infdis/175.1.205.
This murine model of nontypeable (unencapsulated) Haemophilus influenzae (NTHI) bronchopneumonia used organisms bound to mouse fetal lung (MFL) cells as an inoculum. Pretreatment of the mice with 40 microL of 1% formalin 3 days before intranasal instillation of the bacteria was necessary to allow infection. The number of NTHI recovered from the lungs plus trachea on day 7 after instillation was >100 times the number originally inoculated. Later, however, the number of recovered bacteria diminished gradually, and by day 14 it was almost identical to the original inoculum size. Serum IgM also peaked on day 7 after infection, after which IgG increased while IgM decreased. Histologically, bronchoalveolar infiltration of neutrophils was observed on day 3 after inoculation and continued at least for the following 4 days. The present experiment demonstrates that MFL cells can protect bacteria that have invaded the cells from the opsonizing and killing activities of host humoral defense mechanisms.
这种不可分型（无荚膜）流感嗜血杆菌（NTHI）支气管肺炎的小鼠模型使用与小鼠胎肺（MFL）细胞结合的细菌作为接种物。在鼻内滴注细菌前3天用40微升1%福尔马林预处理小鼠对于感染是必要的。滴注后第7天从肺和气管中回收的NTHI数量比最初接种的数量多100倍以上。然而，后来回收的细菌数量逐渐减少，到第14天时几乎与最初接种物的大小相同。血清IgM在感染后第7天也达到峰值，之后IgG增加而IgM减少。组织学上，接种后第3天观察到中性粒细胞的支气管肺泡浸润，并至少持续接下来的4天。本实验表明，MFL细胞可以保护侵入细胞的细菌免受宿主体液防御机制的调理和杀伤活性的影响。
