Rodnina M V, Savelsbergh A, Katunin V I, Wintermeyer W
Institute of Molecular Biology, University of Witten/Herdecke, Germany.
Nature. 1997 Jan 2;385(6611):37-41. doi: 10.1038/385037a0.
Elongation factor G (EF-G) is a GTPase that is involved in the translocation of bacterial ribosomes along messenger RNA during protein biosynthesis. In contrast to current models, EF-G-dependent GTP hydrolysis is shown to precede, and greatly accelerate, the rearrangement of the ribosome that leads to translocation. Domain IV of the EF-G structure is crucial for both rapid translocation and subsequent release of the factor from the ribosome. By coupling the free energy of GTP hydrolysis to translocation, EF-G serves as a motor protein to drive the directional movement of transfer and messenger RNAs on the ribosome.
延伸因子G（EF-G）是一种GTP酶，在蛋白质生物合成过程中参与细菌核糖体沿信使RNA的移位。与当前模型不同的是，EF-G依赖的GTP水解被证明先于导致移位的核糖体重排，并极大地加速了这种重排。EF-G结构的结构域IV对于快速移位以及随后该因子从核糖体上的释放至关重要。通过将GTP水解的自由能与移位相偶联，EF-G作为一种驱动蛋白，推动转运RNA和信使RNA在核糖体上的定向移动。
