一种含有辛德毕斯病毒刺突的塞姆利基森林病毒嵌合体的高效增殖。
Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.
作者信息
Smyth J, Suomalainen M, Garoff H
机构信息
Department of Biosciences at Novum, Huddinge, Sweden.
出版信息
J Virol. 1997 Jan;71(1):818-23. doi: 10.1128/JVI.71.1.818-823.1997.
Abstract
Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed efficient assembly of infectious virus, whereas the reciprocal SIN/SFV(spike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.
摘要
利用Semliki森林病毒（SFV）和辛德毕斯病毒（SIN）的cDNA，我们构建了重组体，其中的刺突基因进行了交换。表达分析表明，SFV/SIN（刺突）RNA指导了感染性病毒的有效组装，而与之相反的SIN/SFV（刺突）RNA则完全无法组装病毒。这显然是由于衣壳与刺突之间的相互作用存在缺陷。
相似文献
1
Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.一种含有辛德毕斯病毒刺突的塞姆利基森林病毒嵌合体的高效增殖。
J Virol. 1997 Jan;71(1):818-23. doi: 10.1128/JVI.71.1.818-823.1997.
2
Semliki Forest virus and Sindbis virus vectors.塞姆利基森林病毒和辛德毕斯病毒载体。
Curr Protoc Hum Genet. 2002 Aug;Chapter 12:Unit 12.2. doi: 10.1002/0471142905.hg1202s33.
3
Low pH-induced pore formation by spike proteins of enveloped viruses.包膜病毒刺突蛋白诱导的低pH值诱导孔形成。
J Gen Virol. 1996 Dec;77 ( Pt 12):3025-32. doi: 10.1099/0022-1317-77-12-3025.
4
The cholesterol requirement for sindbis virus entry and exit and characterization of a spike protein region involved in cholesterol dependence.辛德毕斯病毒进出所需的胆固醇以及参与胆固醇依赖性的刺突蛋白区域的特性
J Virol. 1999 May;73(5):4272-8. doi: 10.1128/JVI.73.5.4272-4278.1999.
5
Adaptive mutations in Sindbis virus E2 and Ross River virus E1 that allow efficient budding of chimeric viruses.辛德毕斯病毒E2和罗斯河病毒E1中的适应性突变，可使嵌合病毒有效出芽。
J Virol. 2000 Mar;74(6):2663-70. doi: 10.1128/jvi.74.6.2663-2670.2000.
6
Sindbis virus replicons and Sindbis virus: assembly of chimeras and of particles deficient in virus RNA.辛德毕斯病毒复制子与辛德毕斯病毒：嵌合体及缺乏病毒RNA的颗粒的组装
J Virol. 1997 Apr;71(4):2819-29. doi: 10.1128/JVI.71.4.2819-2829.1997.
7
Selection of functional 5' cis-acting elements promoting efficient sindbis virus genome replication.促进辛德毕斯病毒基因组高效复制的功能性5'顺式作用元件的筛选。
J Virol. 2004 Jan;78(1):61-75. doi: 10.1128/jvi.78.1.61-75.2004.
8
Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly.Semliki森林病毒假定融合肽中的突变影响刺突蛋白寡聚化和病毒组装。
J Virol. 1995 Apr;69(4):2471-9. doi: 10.1128/JVI.69.4.2471-2479.1995.
9
Variation in homotypic and heterotypic interference by defective interfering viruses derived from different strains of Semliki Forest virus and from Sindbis virus.源自不同株塞姆利基森林病毒和辛德毕斯病毒的缺陷干扰病毒在同型和异型干扰方面的差异。
J Gen Virol. 1984 Jun;65 ( Pt 6):1119-22. doi: 10.1099/0022-1317-65-6-1119.
10
Interactions between PE2, E1, and 6K required for assembly of alphaviruses studied with chimeric viruses.利用嵌合病毒研究甲病毒装配所需的PE2、E1和6K之间的相互作用。
J Virol. 1996 Nov;70(11):7910-20. doi: 10.1128/JVI.70.11.7910-7920.1996.
引用本文的文献
1
Dissecting the Role of E2 Protein Domains in Alphavirus Pathogenicity.剖析E2蛋白结构域在甲病毒致病性中的作用
J Virol. 2015 Dec 16;90(5):2418-33. doi: 10.1128/JVI.02792-15.
2
Lectin-mediated retention of p62 facilitates p62-E1 heterodimerization in endoplasmic reticulum of Semliki Forest virus-infected cells.凝集素介导的p62滞留促进了在感染Semliki森林病毒的细胞内质网中p62-E1异源二聚体的形成。
J Virol. 2003 Jun;77(12):6676-82. doi: 10.1128/jvi.77.12.6676-6682.2003.
3
In vitro assembly of alphavirus cores by using nucleocapsid protein expressed in Escherichia coli.利用在大肠杆菌中表达的核衣壳蛋白进行甲病毒核心的体外组装。
J Virol. 1999 Jul;73(7):5309-19. doi: 10.1128/JVI.73.7.5309-5319.1999.
4
Specific interactions between retrovirus Env and Gag proteins in rat neurons.逆转录病毒包膜蛋白与大鼠神经元中 gag 蛋白之间的特异性相互作用。
J Virol. 1998 Apr;72(4):2832-45. doi: 10.1128/JVI.72.4.2832-2845.1998.
5
Chimeric measles viruses with a foreign envelope.带有外来包膜的嵌合麻疹病毒。
J Virol. 1998 Mar;72(3):2150-9. doi: 10.1128/JVI.72.3.2150-2159.1998.
6
Oligomerization-dependent folding of the membrane fusion protein of Semliki Forest virus.塞姆利基森林病毒膜融合蛋白的寡聚化依赖性折叠
J Virol. 1997 Dec;71(12):9654-63. doi: 10.1128/JVI.71.12.9654-9663.1997.
7
The nucleocapsid-binding spike subunit E2 of Semliki Forest virus requires complex formation with the E1 subunit for activity.塞姆利基森林病毒的核衣壳结合刺突亚基E2需要与E1亚基形成复合物才能发挥活性。
J Virol. 1997 Oct;71(10):7857-65. doi: 10.1128/JVI.71.10.7857-7865.1997.
8
Infectious particles derived from Semliki Forest virus vectors encoding murine leukemia virus envelopes.源自编码鼠白血病病毒包膜的辛德毕斯病毒载体的感染性颗粒。
J Virol. 1997 Sep;71(9):7061-7. doi: 10.1128/JVI.71.9.7061-7067.1997.
9
Sindbis virus replicons and Sindbis virus: assembly of chimeras and of particles deficient in virus RNA.辛德毕斯病毒复制子与辛德毕斯病毒：嵌合体及缺乏病毒RNA的颗粒的组装
J Virol. 1997 Apr;71(4):2819-29. doi: 10.1128/JVI.71.4.2819-2829.1997.
本文引用的文献
1
Aromatic interactions define the binding of the alphavirus spike to its nucleocapsid.芳香相互作用决定了甲病毒刺突蛋白与其核衣壳的结合。
Structure. 1996 May 15;4(5):519-29. doi: 10.1016/s0969-2126(96)00058-5.
2
Deletion analysis of the capsid protein of Sindbis virus: identification of the RNA binding region.辛德毕斯病毒衣壳蛋白的缺失分析：RNA结合区域的鉴定
J Virol. 1993 Mar;67(3):1620-6. doi: 10.1128/JVI.67.3.1620-1626.1993.
3
Membrane protein lateral interactions control Semliki Forest virus budding.膜蛋白侧向相互作用控制塞姆利基森林病毒出芽。
EMBO J. 1994 Mar 1;13(5):1058-64. doi: 10.1002/j.1460-2075.1994.tb06354.x.
4
Incorporation of homologous and heterologous proteins into the envelope of Moloney murine leukemia virus.将同源和异源蛋白整合到莫洛尼鼠白血病病毒包膜中。
J Virol. 1994 Aug;68(8):4879-89. doi: 10.1128/JVI.68.8.4879-4889.1994.
5
The alphaviruses: gene expression, replication, and evolution.甲病毒属：基因表达、复制与进化
Microbiol Rev. 1994 Sep;58(3):491-562. doi: 10.1128/mr.58.3.491-562.1994.
6
A tyrosine-based motif in the cytoplasmic domain of the alphavirus envelope protein is essential for budding.甲病毒包膜蛋白胞质结构域中基于酪氨酸的基序对于出芽至关重要。
EMBO J. 1994 Sep 15;13(18):4204-11. doi: 10.1002/j.1460-2075.1994.tb06740.x.
7
Nucleocapsid and glycoprotein organization in an enveloped virus.包膜病毒中的核衣壳和糖蛋白结构
Cell. 1995 Feb 24;80(4):621-30. doi: 10.1016/0092-8674(95)90516-2.
8
Structure-function relation of the NH2-terminal domain of the Semliki Forest virus capsid protein.辛德毕斯病毒衣壳蛋白氨基末端结构域的结构-功能关系
J Virol. 1995 Mar;69(3):1556-63. doi: 10.1128/JVI.69.3.1556-1563.1995.
9
Low pH induces swiveling of the glycoprotein heterodimers in the Semliki Forest virus spike complex.低pH值诱导塞姆利基森林病毒刺突复合物中糖蛋白异二聚体的旋转。
Cell. 1995 Jun 2;81(5):715-25. doi: 10.1016/0092-8674(95)90533-2.
10
Semliki Forest virus expression system: production of conditionally infectious recombinant particles.塞姆利基森林病毒表达系统：条件性感染重组颗粒的产生。
Biotechnology (N Y). 1993 Aug;11(8):916-20. doi: 10.1038/nbt0893-916.
Zotero 插件