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将同源和异源蛋白整合到莫洛尼鼠白血病病毒包膜中。

Incorporation of homologous and heterologous proteins into the envelope of Moloney murine leukemia virus.

作者信息

Suomalainen M, Garoff H

机构信息

Department of Molecular Biology, Karolinska Institute, Novum, Huddinge, Sweden.

出版信息

J Virol. 1994 Aug;68(8):4879-89. doi: 10.1128/JVI.68.8.4879-4889.1994.

DOI:10.1128/JVI.68.8.4879-4889.1994
PMID:8035486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC236428/
Abstract

The efficiencies with which homologous and heterologous proteins are incorporated into the envelope of Moloney murine leukemia virus (M-MuLV) have been analyzed by utilizing a heterologous, Semliki Forest virus-driven M-MuLV assembly system and quantitative pulse-chase assays. Homologous M-MuLV spike protein was found to be efficiently incorporated into extracellular virus particles when expressed at a relatively low density at the plasma membrane. In contrast, efficient incorporation of heterologous proteins (the spike complex of Semliki Forest virus and a cytoplasmically truncated mutant of the human transferrin receptor) was observed only when these proteins were expressed at high densities at the cell surface. These results imply that homologous and heterologous proteins are incorporated into the M-MuLV envelope via two distinct pathways.

摘要

通过利用异源的、辛德毕斯病毒驱动的莫洛尼鼠白血病病毒（M-MuLV）组装系统和定量脉冲追踪分析，分析了同源和异源蛋白整合到莫洛尼鼠白血病病毒（M-MuLV）包膜中的效率。当同源M-MuLV刺突蛋白在质膜上以相对较低的密度表达时，发现其能有效地整合到细胞外病毒颗粒中。相比之下，仅当这些异源蛋白（辛德毕斯病毒的刺突复合物和人转铁蛋白受体的胞质截短突变体）在细胞表面以高密度表达时，才能观察到它们的有效整合。这些结果表明，同源和异源蛋白通过两条不同的途径整合到M-MuLV包膜中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/da413e323a0e/jvirol00017-0192-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/dccab4c7aeec/jvirol00017-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/298bf616a4c5/jvirol00017-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/2b5951b97152/jvirol00017-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/9439654b8052/jvirol00017-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/831367758cbc/jvirol00017-0189-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/cebc1d3892fd/jvirol00017-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/ca9bda6acc0b/jvirol00017-0190-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/71b3e62568bb/jvirol00017-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/90f180e2b882/jvirol00017-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/da413e323a0e/jvirol00017-0192-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/dccab4c7aeec/jvirol00017-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/298bf616a4c5/jvirol00017-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/2b5951b97152/jvirol00017-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/9439654b8052/jvirol00017-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/831367758cbc/jvirol00017-0189-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/cebc1d3892fd/jvirol00017-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/ca9bda6acc0b/jvirol00017-0190-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/71b3e62568bb/jvirol00017-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/90f180e2b882/jvirol00017-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/236428/da413e323a0e/jvirol00017-0192-b.jpg

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