禽肉瘤病毒和1型人类免疫缺陷病毒整合酶的亚细胞定位
Subcellular localization of avian sarcoma virus and human immunodeficiency virus type 1 integrases.
作者信息
Kukolj G, Jones K S, Skalka A M
机构信息
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
出版信息
J Virol. 1997 Jan;71(1):843-7. doi: 10.1128/JVI.71.1.843-847.1997.
Abstract
The composition and subcellular trafficking of subviral preintegration complexes are reported to vary among the different retroviruses. The process by which the avian sarcoma virus (ASV) preintegration complex gains access to target chromatin remains unknown. Here we report that ASV integrase (IN) expressed as a fusion to beta-galactosidase accumulates in the nuclei of transfected COS-1 cells. In contrast, human immunodeficiency type 1 (HIV-1) IN-beta-galactosidase fusions expressed similarly are predominantly cytoplasmic. To identify the region of ASV IN that specifies nuclear localization, various subdomains of the protein were expressed as beta-galactosidase fusions and their subcellular locations were assessed cytochemically and by indirect immunofluorescence. These analyses showed that the ASV IN protein possesses a functional nuclear localization signal that spans amino acids 206 to 235 and displays limited homology with known nuclear transport signals.
摘要
据报道，不同逆转录病毒的亚病毒整合前复合物的组成和亚细胞运输存在差异。禽肉瘤病毒（ASV）整合前复合物进入靶染色质的过程仍不清楚。在此，我们报道，与β-半乳糖苷酶融合表达的ASV整合酶（IN）在转染的COS-1细胞核中积累。相比之下，以类似方式表达的人类免疫缺陷病毒1型（HIV-1）IN-β-半乳糖苷酶融合蛋白主要位于细胞质中。为了确定ASV IN中指定核定位的区域，该蛋白的各个亚结构域作为β-半乳糖苷酶融合蛋白表达，并通过细胞化学和间接免疫荧光评估其亚细胞定位。这些分析表明，ASV IN蛋白具有一个功能性核定位信号，该信号跨越氨基酸206至235，与已知的核运输信号显示出有限的同源性。
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本文引用的文献
1
Portals of entry: uncovering HIV nuclear transport pathways.进入途径：揭示HIV核转运途径
Trends Cell Biol. 1996 Jan;6(1):9-15. doi: 10.1016/0962-8924(96)81032-4.
2
Retroviral integrase, putting the pieces together.逆转录病毒整合酶：整合过程解析
J Biol Chem. 1996 Aug 16;271(33):19633-6. doi: 10.1074/jbc.271.33.19633.
3
Conserved sequences in the carboxyl terminus of integrase that are essential for human immunodeficiency virus type 1 replication.整合酶羧基末端的保守序列，对于1型人类免疫缺陷病毒复制至关重要。
J Virol. 1996 Jan;70(1):651-7. doi: 10.1128/JVI.70.1.651-657.1996.
4
Integration of murine leukemia virus DNA depends on mitosis.鼠白血病病毒DNA的整合依赖于有丝分裂。
EMBO J. 1993 May;12(5):2099-108. doi: 10.1002/j.1460-2075.1993.tb05858.x.
5
Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron.如果env区域未被定义为内含子，人类免疫缺陷病毒env表达将变得不依赖于Rev。
J Virol. 1994 Feb;68(2):951-8. doi: 10.1128/JVI.68.2.951-958.1994.
6
Passage through mitosis is required for oncoretroviruses but not for the human immunodeficiency virus.致肿瘤逆转录病毒需要经历有丝分裂，而人类免疫缺陷病毒则不需要。
J Virol. 1994 Jan;68(1):510-6. doi: 10.1128/JVI.68.1.510-516.1994.
7
Nuclear localization signals (NLS).核定位信号（NLS）。
Crit Rev Eukaryot Gene Expr. 1993;3(3):193-227.
8
A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells.HIV-1基质蛋白内的一个核定位信号，它控制非分裂细胞的感染。
Nature. 1993 Oct 14;365(6447):666-9. doi: 10.1038/365666a0.
9
The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells.人类免疫缺陷病毒1型的Vpr蛋白影响病毒核酸在非分裂宿主细胞中的核定位。
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7311-5. doi: 10.1073/pnas.91.15.7311.
10
The nuclear localization signal of the matrix protein of human immunodeficiency virus type 1 allows the establishment of infection in macrophages and quiescent T lymphocytes.1型人类免疫缺陷病毒基质蛋白的核定位信号可促使其在巨噬细胞和静止T淋巴细胞中建立感染。
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6992-6. doi: 10.1073/pnas.91.15.6992.
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