Roe T, Reynolds T C, Yu G, Brown P O
Department of Biochemistry, Stanford University, CA 94305.
EMBO J. 1993 May;12(5):2099-108. doi: 10.1002/j.1460-2075.1993.tb05858.x.
In synchronized rat or mouse cells infected with Moloney murine leukemia virus (MLV), integration of viral DNA and production of viral proteins occur only after the cells traverse mitosis. Integration is blocked when cells are prevented from progressing through mitosis. Viral nucleoprotein complexes isolated from arrested cells contain full-length viral DNA and can integrate this viral DNA in vitro, showing that the block to integration in arrested cells is not due to a lack of mature integration machinery. When infected cells traverse mitosis, there is a sharp increase in nuclear accumulation of viral DNA. The dependence of integration on mitosis may therefore be due to a requirement for mitosis and nuclear envelope breakdown for entry of the viral integration complex into the nucleus.
在感染莫洛尼鼠白血病病毒（MLV）的同步化大鼠或小鼠细胞中，病毒DNA的整合和病毒蛋白的产生仅在细胞经历有丝分裂后才会发生。当细胞被阻止进行有丝分裂时，整合被阻断。从停滞细胞中分离出的病毒核蛋白复合物含有全长病毒DNA，并且可以在体外整合这种病毒DNA，这表明停滞细胞中整合的阻断并非由于缺乏成熟的整合机制。当感染的细胞经历有丝分裂时，病毒DNA的核积累会急剧增加。因此，整合对有丝分裂的依赖性可能是由于有丝分裂和核膜破裂是病毒整合复合物进入细胞核所必需的。
