用γ逆转录病毒衍生载体转导非分裂期人类巨噬细胞。
Transduction of nondividing human macrophages with gammaretrovirus-derived vectors.
作者信息
Jarrosson-Wuilleme Loraine, Goujon Caroline, Bernaud Jeanine, Rigal Dominique, Darlix Jean-Luc, Cimarelli Andrea
机构信息
LaboRetro, INSERM U412, Ecole Normale Supérieure de Lyon, IFR 128 BioSciences Lyon-Gerland, 46 Allée d'Italie, 69364 Lyon, France.
出版信息
J Virol. 2006 Feb;80(3):1152-9. doi: 10.1128/JVI.80.3.1152-1159.2006.
Abstract
It is commonly accepted that infection of nondividing cells by gammaretroviruses such as the murine leukemia viruses is inefficient due to their inability to cross the nuclear envelope barrier. Challenging this notion, we now show that human nondividing macrophages display a specific window of susceptibility to transduction with a Friend murine leukemia virus (F-MLV)-derived vector during their differentiation from monocytes. This finding suggests that factors other than the nuclear membrane govern permissiveness to gammaretroviral infection and raises the possibility of using the macrophage tropism of F-MLV in gene therapy.
摘要
人们普遍认为，诸如鼠白血病病毒之类的γ逆转录病毒感染非分裂细胞的效率低下，因为它们无法穿过核膜屏障。为了挑战这一观念，我们现在表明，人类非分裂巨噬细胞在从单核细胞分化的过程中，对源自Friend鼠白血病病毒（F-MLV）的载体转导表现出特定的易感窗口期。这一发现表明，除核膜外的其他因素决定了对γ逆转录病毒感染的易感性，并增加了在基因治疗中利用F-MLV的巨噬细胞嗜性的可能性。
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