子痫前期患者绒毛外滋养层细胞上人白细胞抗原G（HLA-G）表达的改变：用抗HLA-G特异性抗体“87G”和抗细胞角蛋白抗体“CAM5.2”进行免疫组织学证明
Altered expression of human leukocyte antigen G (HLA-G) on extravillous trophoblasts in preeclampsia: immunohistological demonstration with anti-HLA-G specific antibody "87G" and anti-cytokeratin antibody "CAM5.2".
作者信息
Hara N, Fujii T, Yamashita T, Kozuma S, Okai T, Taketani Y
机构信息
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Am J Reprod Immunol. 1996 Dec;36(6):349-58. doi: 10.1111/j.1600-0897.1996.tb00185.x.
PROBLEM
Human leukocyte antigen-G (HLA-G) is suggested to be at play in the materno-fetal immune relationship during pregnancy. In the light of current concept that disruption of the materno-fetal immune relationship could account for several complications of pregnancy, including preeclampsia, we asked whether the expression of HLA-G protein on the trophoblasts is altered in preeclampsia.
METHOD
The presence of HLA-G protein in the extravillous trophoblasts in placenta obtained from five preeclamptic patients and seven uncomplicated pregnant women was determined by means of an immunohistochemical technique.
RESULTS
All of the extravillous trophoblasts, which were stained for cytokeratin, were stained for HLA-G protein in every woman with an uncomplicated pregnancy. In contrast, clusters of extravillous trophoblasts were insularly devoid of the staining for HLA-G in all the preeclamptic patients.
CONCLUSION
The attenuated expression of HLA-G protein on the extravillous trophoblasts could be at play in the pathophysiology of preeclampsia.
问题
人类白细胞抗原G（HLA - G）被认为在孕期母胎免疫关系中发挥作用。鉴于目前的观念认为母胎免疫关系的破坏可能导致多种妊娠并发症，包括先兆子痫，我们探讨了先兆子痫患者胎盘绒毛外滋养层细胞上HLA - G蛋白的表达是否发生改变。
方法
采用免疫组化技术，检测了5例先兆子痫患者和7例正常孕妇胎盘绒毛外滋养层细胞中HLA - G蛋白的表达情况。
结果
在所有正常妊娠的女性中，所有细胞角蛋白染色阳性的绒毛外滋养层细胞均有HLA - G蛋白染色。相反，在所有先兆子痫患者中，绒毛外滋养层细胞簇均呈岛状缺乏HLA - G染色。
结论
绒毛外滋养层细胞上HLA - G蛋白表达减弱可能在先兆子痫的病理生理过程中起作用。
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