Kawaguchi E, Minamide W, Mori H, Igimi H
Shionogi Biomedical Laboratories, Shionogi & Co., Ltd.
Kansenshogaku Zasshi. 1996 Nov;70(11):1147-53. doi: 10.11150/kansenshogakuzasshi1970.70.1147.
The species, mecA gene, beta-lactamase activity, the ability of slime formation and drug susceptibilities of 386 strains of staphylococci which were isolated from blood in our laboratory were studied. The coagulase typing of each strains identified as S. aureus was also determined. These 386 strains consisted of various species, e.g., S. aureus, S. epidermidis, S. capitis, S. caprae, S. hominis, S. simulans, S. haemolyticus, and S. lugdunensis. mecA (methicillin-resistant gene) was detected in 84 (67.7%) of 124 S. aureus and 195 (75.3%) of 259 CNS, but there was no statistical difference. However mecA positive rate was higher in S. epidermidis and S. caprae, lower in S. hominis compared with S. aureus, S. lugdunensis having mecA has not been reported, but one of two our S. lugdunensis strains had mecA. The positive rate (77.4%: 65/84) of beta-lactamase of methicillin-resistant Staphylococcus aureus (MRSA) was lower than that (95.5%: 187/195) of methicillin-resistant coagulase-negative staphylococci (MRCNS). Concerning the ability of slime formation, CNS had higher positive rate, especially in case of MRCNS (46.2%: 84/182) than S. aureus. On the other hand, MRCNS showed a tendency to be less resistant to some antimicrobials than MRSA. Especially against cephalothin (CET), the resistant rate of MRSA and MRCNS were 86.9% and 5.6%, respectively. Among the main species of MRCNS, S. capitis and S. caprae were more resistant than S. epidermidis and S. simulans to cefaclor (CCL), cefmetazole (CMZ), flomoxef (FMOX) and fosfomycin (FOM). Hereafter, one should be careful about infectious disease caused by CNS especially in immuno-compromised host, because many species of CNS have higher positive rate of beta-lactamase and slime formation than S. aureus beside mecA.
对本实验室从血液中分离出的386株葡萄球菌的菌种、mecA基因、β-内酰胺酶活性、形成黏液的能力及药敏情况进行了研究。对 Identification was also determined. These 386 strains consisted of various species, e.g., S. aureus, S. epidermidis, S. capitis, S. caprae, S. hominis, S. simulans, S. haemolyticus, and S. lugdunensis. mecA（耐甲氧西林基因）在124株金黄色葡萄球菌中的84株（67.7%）及259株凝固酶阴性葡萄球菌中的195株（75.3%）被检测到，但无统计学差异。然而，表皮葡萄球菌和山羊葡萄球菌中的mecA阳性率较高，人葡萄球菌中的mecA阳性率低于金黄色葡萄球菌，尚未有关于路邓葡萄球菌携带mecA的报道，但我们的2株路邓葡萄球菌中有1株携带mecA。耐甲氧西林金黄色葡萄球菌（MRSA）的β-内酰胺酶阳性率（77.4%：65/84）低于耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）（95.5%：187/195）。关于形成黏液的能力，凝固酶阴性葡萄球菌的阳性率较高，尤其是MRCNS（46.2%：84/182）高于金黄色葡萄球菌。另一方面，MRCNS对某些抗菌药物的耐药性有低于MRSA的趋势。尤其是对头孢噻吩（CET），MRSA和MRCNS的耐药率分别为86.9%和5.6%。在MRCNS的主要菌种中，头状葡萄球菌和山羊葡萄球菌对头孢克洛（CCL）、头孢美唑（CMZ）、氟氧头孢（FMOX）和磷霉素（FOM）的耐药性高于表皮葡萄球菌和模仿葡萄球菌。此后，应特别关注凝固酶阴性葡萄球菌引起的感染性疾病，尤其是在免疫功能低下的宿主中，因为除mecA外，许多凝固酶阴性葡萄球菌菌种的β-内酰胺酶阳性率和形成黏液的能力高于金黄色葡萄球菌。 每株鉴定为金黄色葡萄球菌的菌株也进行了凝固酶分型。这386株菌株包括多种菌种，如金黄色葡萄球菌、表皮葡萄球菌、头状葡萄球菌、山羊葡萄球菌、人葡萄球菌、模仿葡萄球菌、溶血葡萄球菌和路邓葡萄球菌。
