UNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, Brazil; UNOESTE-Universidade Oeste Paulista, Departamento de Microbiologia, São Paulo, SP, Brazil.
UNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, Brazil.
Braz J Infect Dis. 2020 Mar-Apr;24(2):160-169. doi: 10.1016/j.bjid.2020.01.003. Epub 2020 Feb 19.
The increasing rates of nosocomial infection associated with coagulase-negative staphylococci (CoNS) were the rationale for this study, aiming to categorize oxacillin-resistant CoNS species recovered from blood culture specimens of inpatients at the UNESP Hospital das Clínicas in Botucatu, Brazil, over a 20-year period, and determine their sensitivity to other antimicrobial agents. The mecA gene was detected in 222 (74%) CoNS samples, and the four types of staphylococcal chromosomal cassette mec (SCCmec) were characterized in 19.4%, 3.6%, 54.5%, and 14.4% of specimens, respectively, for types I, II, III, and IV. Minimal inhibitory concentration (MIC) values to inhibit 50% (MIC50) and 90% (MIC90) of specimens were, respectively, 2 and >256μL/mL for oxacillin, 1.5 and 2μL/mL for vancomycin, 0.25 and 0.5μL/mL for linezolid, 0.094 and 0.19μL/mL for daptomycin, 0.19 and 0.5μL/mL for quinupristin/dalfopristin, and 0.125 and 0.38μL/mL for tigecycline. Resistance to oxacillin and tigecycline and intermediate resistance to quinupristin/dalfopristin were observed. Eight (2.7%) of all 300 CoNS specimens studied showed reduced susceptibility to vancomycin. Results from this study show high resistance rates of CoNS to antimicrobial agents, reflecting the necessity of using these drugs judiciously and controlling nosocomial dissemination of these pathogens.
凝固酶阴性葡萄球菌（CoNS）引起的医院感染率不断上升，这是本研究的基础。本研究旨在对巴西博图卡图 UNESP 医院临床系住院患者血培养标本中分离的耐苯唑西林凝固酶阴性葡萄球菌进行分类，并确定其对其他抗菌药物的敏感性。在 222 份（74%）CoNS 样本中检测到 mecA 基因，19.4%、3.6%、54.5%和 14.4%的样本分别携带 I 型、II 型、III 型和 IV 型葡萄球菌染色体盒 mec（SCCmec）。最低抑菌浓度（MIC）值抑制 50%（MIC50）和 90%（MIC90）的标本分别为 2 和>256μL/mL 用于苯唑西林，1.5 和 2μL/mL 用于万古霉素，0.25 和 0.5μL/mL 用于利奈唑胺，0.094 和 0.19μL/mL 用于达托霉素，0.19 和 0.5μL/mL 用于奎奴普丁/达福普汀，0.125 和 0.38μL/mL 用于替加环素。观察到对苯唑西林和替加环素的耐药性和对奎奴普丁/达福普汀的中介耐药性。在所研究的 300 份 CoNS 标本中，有 8 份（2.7%）对万古霉素表现出低敏感性。本研究结果显示 CoNS 对抗菌药物的耐药率较高，反映出需要合理使用这些药物，并控制这些病原体的医院传播。
