Avallone L, Lombardi P, Florio S, d'Angelo A, Bogin E
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Università degli Studi di Napoli Federico II, Napoli, Italy.
Eur J Clin Chem Clin Biochem. 1996 Dec;34(12):961-4. doi: 10.1515/cclm.1996.34.12.961.
The electrophoretic patterns of the serum enzymes lactate dehydrogenase and creatine kinase from water buffalo calves are described. Differences in total activities as well as their relative distribution were seen at ages ranging from 1 to 10 weeks. While total lactate dehydrogenase activity increased by over 100%, total creatine kinase increased by almost 400%. The relative activities of lactate dehydrogenase 1 and 5 decreased with age. Lactate dehydrogenase 2 and 3 increased and lactate dehydrogenase 4 did not change. In relation to creatine kinase, the prevalent isoenzyme was creatine kinase-MM, but it's relative activity gradually decreased in comparison to the other two isoenzymes (creatine kinase-MB and creatine kinase-BB). Creatine kinase-BB was completely absent until the 3rd week of age. The percentage modifications of creatine kinase isoenzymes were correlated to age. The results suggest that isoenzymatic separation and characterization of lactate dehydrogenase and creatine kinase in relation to the various tissues can significantly contribute to the diagnosis of diseases which are linked to tissue damage.
本文描述了水牛犊血清中乳酸脱氢酶和肌酸激酶的电泳图谱。在1至10周龄范围内，观察到这两种酶的总活性及其相对分布存在差异。乳酸脱氢酶的总活性增加了100%以上，而肌酸激酶的总活性几乎增加了400%。乳酸脱氢酶1和5的相对活性随年龄增长而降低，乳酸脱氢酶2和3增加，乳酸脱氢酶4不变。关于肌酸激酶，主要的同工酶是肌酸激酶-MM，但与其他两种同工酶（肌酸激酶-MB和肌酸激酶-BB）相比，其相对活性逐渐降低。直到3周龄时，肌酸激酶-BB才完全消失。肌酸激酶同工酶的百分比变化与年龄相关。结果表明，乳酸脱氢酶和肌酸激酶同工酶相对于各种组织的分离和表征，可显著有助于诊断与组织损伤相关的疾病。
