Ichihara Elephant Kingdom Zoological Park, Chiba, Japan.
Department of Veterinary Science, School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
J Vet Med Sci. 2023 May 3;85(5):578-583. doi: 10.1292/jvms.22-0550. Epub 2023 Mar 14.
Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) isoenzymes were evaluated in nine zoo-managed Asian elephants (Elephas maximus) using a commercial agarose gel electrophoresis (AGE) kit. CK was separated into two major fractions, CK-BB and CK-MM, along with a small fraction of macroenzyme-CK type 2 (mCK2); CK-MM was the largest fraction. LDH was separated into five fractions (LDH1-5); LDH3 was the largest fraction. Age was negatively and positively correlated with the percentages of CK-BB and CK-MM, respectively, and negatively correlated with CK-BB and mCK2 activities. These results indicate that an AGE kit can be used to evaluate CK and LDH isoenzymes. Routine isoenzyme testing may enable early detection of disease and physiological changes.
采用商用琼脂糖凝胶电泳（AGE）试剂盒检测了 9 头圈养亚洲象（Elephas maximus）血清肌酸激酶（CK）和乳酸脱氢酶（LDH）同工酶的水平。CK 分离为两个主要部分，CK-BB 和 CK-MM，以及少量巨 CK2 型（mCK2）；CK-MM 是最大的部分。LDH 分离为五个部分（LDH1-5）；LDH3 是最大的部分。年龄与 CK-BB 和 CK-MM 的百分比呈负相关和正相关，与 CK-BB 和 mCK2 活性呈负相关。这些结果表明，AGE 试剂盒可用于评估 CK 和 LDH 同工酶。常规同工酶检测可能有助于早期发现疾病和生理变化。
