Tsvetkova Ts M
Vet Med Nauki. 1977;14(1):42-6.
Described is a thin-layer chromatography method for the demonstration and identification of the cumarin derivatives cumaphos and warfarin. Tested were five solvents and six developers. Best results were obtained by means of Silica gel plates, the toluol-aceton solvent (85:15), and the developer of a diazosalt 0.4% in an alcohol 20% sodiumhydroxide. Warfarin and cumaphos are demonstrated with the appearance of yellow, resp. orange spots on a white background, the RF values being 0.37 and 0.85, and sensitivity as regards warfarin 0.5 microgram and cumaphos 0.2 microgram.
描述了一种用于展示和鉴定香豆素衍生物谷硫磷和华法林的薄层色谱法。测试了五种溶剂和六种显色剂。使用硅胶板、甲苯 - 丙酮溶剂（85:15）以及在20%氢氧化钠乙醇溶液中含0.4%重氮盐的显色剂可获得最佳结果。华法林和谷硫磷分别在白色背景上呈现黄色、橙色斑点，比移值分别为0.37和0.85，华法林的灵敏度为0.5微克，谷硫磷的灵敏度为0.2微克。
