由基因抑制子揭示的发育信号转导途径。
Developmental signal transduction pathways uncovered by genetic suppressors.
作者信息
Shaulsky G, Escalante R, Loomis W F
机构信息
Department of Biology, University of California at San Diego, La Jolla 92093, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15260-5. doi: 10.1073/pnas.93.26.15260.
Abstract
We have found conditions for saturation mutagenesis by restriction enzyme mediated integration that result in plasmid tagging of disrupted genes. Using this method we selected for mutations in genes that act at checkpoints downstream of the intercellular signalling system that controls encapsulation in Dictyostelium discoideum. One of these genes, mkcA, is a member of the mitogen-activating protein kinase cascade family while the other, regA is a novel bipartite gene homologous to response regulators in one part and to cyclic nucleotide phosphodiesterases in the other part. Disruption of either of these genes results in partial suppression of the block to spore formation resulting from the loss of the prestalk genes, tagB and tagC. The products of the tag genes have conserved domains of serine protease attached to ATP-driven transporters, suggesting that they process and export peptide signals. Together, these genes outline an intercellular communication system that coordinates organismal shape with cellular differentiation during development.
摘要
我们已经找到了通过限制酶介导的整合进行饱和诱变的条件，该条件可导致对破坏基因进行质粒标记。利用这种方法，我们筛选了在盘基网柄菌中控制包囊化的细胞间信号系统下游检查点起作用的基因突变。其中一个基因mkcA是丝裂原活化蛋白激酶级联家族的成员，而另一个基因regA是一个新的二分体基因，一部分与应答调节因子同源，另一部分与环核苷酸磷酸二酯酶同源。破坏这两个基因中的任何一个都会部分抑制因前柄基因tagB和tagC缺失而导致的孢子形成障碍。tag基因的产物具有与ATP驱动转运蛋白相连的丝氨酸蛋白酶保守结构域，这表明它们加工并输出肽信号。这些基因共同勾勒出一个细胞间通讯系统，该系统在发育过程中协调生物体形态与细胞分化。
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本文引用的文献
1
Dual Effects of cAMP on the Stability of Prespore Vesicles and 8-bromo cAMP-enhanced Maturation of Spore and Stalk Cells of Dictyostelium discoideum: (prespore vesicle/monolayer sheets/cAMP/8-bromo cAMP/Dictyostelium).环磷酸腺苷（cAMP）对盘基网柄菌前孢子囊泡稳定性的双重影响以及8-溴环磷酸腺苷（8-bromo cAMP）增强盘基网柄菌孢子和柄细胞成熟的作用：（前孢子囊泡/单层片/cAMP/8-溴环磷酸腺苷/盘基网柄菌）
Dev Growth Differ. 1988 Oct;30(5):573-587. doi: 10.1111/j.1440-169X.1988.00573.x.
2
Initial cell type divergence in Dictyostelium is independent of DIF-1.盘基网柄菌中最初的细胞类型分化独立于DIF-1。
Dev Biol. 1996 Mar 15;174(2):214-20. doi: 10.1006/dbio.1996.0067.
3
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.CTR1是拟南芥乙烯反应途径的负调控因子，编码raf蛋白激酶家族的一个成员。
Cell. 1993 Feb 12;72(3):427-41. doi: 10.1016/0092-8674(93)90119-b.
4
A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae.一个显性截短等位基因鉴定出一个基因STE20，它编码酿酒酵母交配所需的一种假定蛋白激酶。
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):452-6. doi: 10.1073/pnas.90.2.452.
5
The structure of a bovine lung cGMP-binding, cGMP-specific phosphodiesterase deduced from a cDNA clone.从一个cDNA克隆推导的牛肺cGMP结合、cGMP特异性磷酸二酯酶的结构。
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6
Cell type regulation in response to expression of ricin A in Dictyostelium.盘基网柄菌中响应蓖麻毒素A表达的细胞类型调控
Dev Biol. 1993 Nov;160(1):85-98. doi: 10.1006/dbio.1993.1288.
7
Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators.拟南芥乙烯反应基因ETR1：其产物与双组分调节因子的相似性
Science. 1993 Oct 22;262(5133):539-44. doi: 10.1126/science.8211181.
8
A two-component system that regulates an osmosensing MAP kinase cascade in yeast.一种调节酵母中渗透压感应丝裂原活化蛋白激酶级联反应的双组分系统。
Nature. 1994 May 19;369(6477):242-5. doi: 10.1038/369242a0.
9
STE6, the yeast a-factor transporter.STE6，酵母a因子转运蛋白。
Semin Cell Biol. 1993 Feb;4(1):17-27. doi: 10.1006/scel.1993.1003.
10
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Mol Cell Biochem. 1993 Nov;127-128:239-53. doi: 10.1007/BF01076775.
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