García-López P, Pérez-Urizar J, Ibarra A, Grijalva I, Madrazo I, Flores-Murrieta F, Castañeda-Hernández G, Guízar-Sahagún G
Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F.
Arch Med Res. 1996 Winter;27(4):453-7.
Two strains of rats, Sprague-Dawley and Wistar, were assayed in order to determine which strain is the more suitable experimental model for the study of pharmacokinetic alterations induced by spinal cord injury. Animals were submitted to spinal cord contusion at the T8-T9 level by the weight drop method. A single acetaminophen oral dose (100 mg/ kg) was administered 24 h after injury and blood samples were drawn for a period of 4 h. Acetaminophen concentration in whole blood was determined by high performance liquid chromatography and pharmacokinetic parameters were estimated. For both strains, Cmax and AUC were significantly lower, whereas tmax remained unchanged, in injured animals compared to sham-injured controls. Circulating acetaminophen concentrations were higher; therefore, pharmacokinetic alterations were more easily discerned, in Sprague-Dawley than in Wistar rats. It is concluded that the Sprague-Dawley strain is a more suitable model for the study of pharmacokinetic alterations induced by spinal cord injury.
为了确定哪种品系的大鼠更适合作为研究脊髓损伤诱导的药代动力学改变的实验模型，对两种品系的大鼠，即斯普拉格-道利大鼠和Wistar大鼠进行了测定。通过重物坠落法在T8-T9水平对动物进行脊髓挫伤。在损伤后24小时给予一次对乙酰氨基酚口服剂量（100毫克/千克），并采集4小时的血样。通过高效液相色谱法测定全血中对乙酰氨基酚的浓度，并估算药代动力学参数。与假手术对照组相比，两种品系的损伤动物的Cmax和AUC均显著降低，而tmax保持不变。斯普拉格-道利大鼠的循环对乙酰氨基酚浓度更高；因此，与Wistar大鼠相比，药代动力学改变在斯普拉格-道利大鼠中更容易辨别。得出的结论是，斯普拉格-道利品系是研究脊髓损伤诱导的药代动力学改变的更合适模型。
