阴道毛滴虫菌株在PEHPS培养基和TYI-S-33培养基中的生长情况相当。

Comparable growth of a Trichomonas vaginalis strain in PEHPS and TYI-S-33 media.

作者信息

Castro-Garza J, Anaya-Velázquez F, Said-Fernández S, González-Garza M T

机构信息

División de Biología Celular y Molecular, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México.

出版信息

Arch Med Res. 1996 Winter;27(4):567-9.

PMID:8987196

Abstract

PEHPS medium, developed for axenic cultivation of Entamoeba histolytica and E. invadens, was also capable of supporting the growth of a Trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. The logarithmic growth phase in PEHPS or in TYI-S-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in PEHPS medium showed no significant differences with those obtained in TYI-S-33 under similar culture conditions. Accordingly, PEHPS medium might be used for the axenic cultivation of T. vaginalis.

摘要

为溶组织内阿米巴和侵袭内阿米巴的无菌培养而研发的PEHPS培养基，也能够支持阴道毛滴虫菌株的生长，接种量为每毫升1至100个滴虫。在PEHPS或TYI-S-33培养基中的对数生长期持续72小时；在相似培养条件下，PEHPS培养基中的产量（3.33±0.56×10⁶个滴虫/毫升）、倍增时间（4.27小时）、倍增次数（16.85）或增长比率（33328）与在TYI-S-33培养基中获得的结果相比，无显著差异。因此，PEHPS培养基可用于阴道毛滴虫的无菌培养。