[不同培养基对阴道毛滴虫体外培养的比较作用]
[Comparative effect of different media in in vitro cultivation of Trichomonas vaginalis].
作者信息
Geng Zhi-hui, Han Shu-mei, Liu Li, Shi Yu-lu, Li Shu-hong
机构信息
Department of the Third Centre, Norman Bethune University of Medical Sciences, Changchun 130021.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2002;20(1):42-4.
OBJECTIVE
To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T. vaginalis.
METHODS
An isolate of T. vaginalis from clinical specimens was cultivated in three different media with initial inoculation of 9.0 x 10(4)/ml under pH 5.6.
RESULTS
There was distinct difference after 96 h incubation in the cumulative harvest of T. vaginalis. The highest harvest was received in cysteine/liver/peptone/maltose medium, followed by the liver/peptone/maltose medium and soybean/liver/peptone/maltose medium.
CONCLUSION
The cysteine/liver/peptone/maltose medium may be a suitable environment for in vitro multiplication of T. vaginalis.
目的
研究阴道毛滴虫体外培养的最佳条件，以获得更好的虫体收获量。
方法
从临床标本中分离出的一株阴道毛滴虫，接种浓度为9.0×10⁴/ml，在pH 5.6条件下，于三种不同培养基中培养。
结果
培养96小时后，阴道毛滴虫的累积收获量存在显著差异。半胱氨酸/肝脏/蛋白胨/麦芽糖培养基中收获量最高，其次是肝脏/蛋白胨/麦芽糖培养基和大豆/肝脏/蛋白胨/麦芽糖培养基。
结论
半胱氨酸/肝脏/蛋白胨/麦芽糖培养基可能是阴道毛滴虫体外增殖的适宜环境。
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