去卷积后的电阻抗谱跟踪不同的细胞形态变化。

Deconvolved electrical impedance spectra track distinct cell morphology changes.

作者信息

McRae D A, Esrick M A

机构信息

Department of Radiation Medicine, Lombardi Cancer Center, Georgetown University Medical Center, Washington, D.C. 20007, USA.

出版信息

IEEE Trans Biomed Eng. 1996 Jun;43(6):607-18. doi: 10.1109/10.495280.

DOI:10.1109/10.495280

PMID:8987265

Abstract

A two-component Cole-Cole model was used to obtain statistically significant fits to 100-Hz-10-MHz impedance data for EMT-6 mouse tumors during the progressive histological changes induced by hyperthermia. The resulting fitting parameters were used to deconvolute and reconstruct the two dispersions which confer the predominant impedance features to this tissue. The time-dependent changes of these two dispersions were correlated with the concurrent, heat-induced morphological changes of the tumors' cells. The higher frequency dispersion (fc approximately 1 MHz) was identified with a Maxwell-Wagner relaxation process linked to the overall volume response of the cells. The lower frequency dispersion (fc approximately 10 kHz) represented an alpha-relaxation associated with the surface morphology and integrity of the plasma membranes. Thus, two aspects of the characteristic cellular damage sequence in these tumors were found to be separately discernable and trackable in real-time using the impedance data.

摘要

使用双组分Cole-Cole模型对高温诱导的EMT-6小鼠肿瘤在组织学渐进性变化过程中的100 Hz至10 MHz阻抗数据进行统计学上显著的拟合。所得拟合参数用于解卷积和重建赋予该组织主要阻抗特征的两个色散。这两个色散随时间的变化与肿瘤细胞同时发生的热诱导形态变化相关。高频色散（fc约为1 MHz）与与细胞总体积响应相关的Maxwell-Wagner弛豫过程相关。低频色散（fc约为10 kHz）代表与质膜表面形态和完整性相关的α弛豫。因此，发现这些肿瘤中特征性细胞损伤序列的两个方面可以使用阻抗数据实时分别辨别和追踪。