使用免疫聚合酶链反应检测细菌抗原。

Detection of bacterial antigens using immuno-PCR.

作者信息

Kakizaki E, Yoshida T, Kawakami H, Oseto M, Sakai T, Sakai M

机构信息

Faculty of Agriculture, Miyazaki University, Japan.

出版信息

Lett Appl Microbiol. 1996 Aug;23(2):101-3. doi: 10.1111/j.1472-765x.1996.tb00040.x.

DOI:10.1111/j.1472-765x.1996.tb00040.x

PMID:8987448

Abstract

A new and very sensitive antigen detection technique, immuno-polymerase chain reaction (immuno-PCR), was developed. This method is basically similar to the enzyme-linked immunosorbent assay which detects an antigen-antibody reaction, but instead of an enzyme being conjugated to an antibody, a DNA fragment is used and this DNA can be amplified by PCR. We applied this method to the detection of the fish pathogen, Pasteurella piscicida, in naturally infected yellowtail. Using immuno-PCR, 3.4 cfu ml-1 of bacteria could be detected. In comparison, ELISA detected only 3.4 x 10(4) cfu ml-1. Immuno-PCR is a powerful method for detection of pathogens in host tissues.

摘要

一种新的高灵敏度抗原检测技术——免疫聚合酶链反应（immuno-PCR）被开发出来。该方法与检测抗原-抗体反应的酶联免疫吸附测定法基本相似，但不是将酶与抗体结合，而是使用一个DNA片段，并且这个DNA可以通过聚合酶链反应进行扩增。我们将该方法应用于自然感染的黄尾鱼中鱼类病原体杀鱼巴斯德氏菌的检测。使用免疫聚合酶链反应，可以检测到每毫升3.4个菌落形成单位的细菌。相比之下，酶联免疫吸附测定法仅能检测到每毫升3.4×10⁴个菌落形成单位。免疫聚合酶链反应是检测宿主组织中病原体的一种强大方法。