Pestchanker L J, Roberts S C, Shuler M L
School of Chemical Engineering, Cornell University, Ithaca, New York 14853-5201, USA.
Enzyme Microb Technol. 1996 Sep;19(4):256-60. doi: 10.1016/0141-0229(95)00243-x.
Suspension cultures of Taxus cuspidata were grown in shake flasks and Wilson-type reactors where bubbled air provided both agitation and mixing. Taxol titers of 22 mg l-1 were achieved for both configurations in 20 days for a volumetric productivity of 1.1 mg l-1 day-1. This productivity is many-fold higher than reported for other Taxus sp. suspension cultures. Taxol was released to the extracellular medium as it was produced with little intracellular retention (< or = 10%). Taxol production occurred during the last seven days of the cultivation period and was not growth-associated. Although the same taxol titers could be obtained in both reactor types, nutrient uptake rates were faster in the Wilson-type reactor than in shake flasks. Formation of a growth ring in the Wilson-type reactor reduced measured cell mass yields.
东北红豆杉悬浮培养物在摇瓶和威尔逊型反应器中培养，鼓入的空气起到搅拌和混合作用。两种培养装置在20天内紫杉醇滴度均达到22 mg l-1，体积产率为1.1 mg l-1天-1。该产率比其他红豆杉属悬浮培养物报道的产率高出许多倍。紫杉醇产生后释放到细胞外培养基中，细胞内保留量很少（≤10%）。紫杉醇的产生发生在培养期的最后七天，与生长无关。虽然两种反应器类型都能获得相同的紫杉醇滴度，但威尔逊型反应器中的营养物质吸收速率比摇瓶中更快。威尔逊型反应器中生长环的形成降低了测得的细胞质量产率。
