Tachinbana Sanro, Muranaka Toshio, Itoh Kazutaka
Department of Applied Bioscience, Faculty of Agriculture, Ehime University, Tarumi 3-5-7, Matsuyama, Ehime 790-8566, Japan.
Pak J Biol Sci. 2007 Sep 1;10(17):2856-61. doi: 10.3923/pjbs.2007.2856.2861.
To stimulate the production of taxol (paclitaxel) by cell suspension cultures of Taxus cuspidata var. nana, two kinds of elicitors and a biogenetic precursor were used in F4G4 culture medium. Paclitaxel production was enhanced by each elicitor as well as by the biogenetic precursor and by a combination of the two elicitors or one elicitor and the biogenetic precursor. The amount ofpaclitaxel produced (16.6 mg L(-1)) was greatest when the cell suspension cultures were conducted in F4G4 medium containing chito-heptaose (8 mg L(-1)) and jasmonic acid (21 mg L(-1)). The productivity was enhanced 4.1 fold compared to the control. The amount of paclitaxel produced was increased by supplying air to the cultures, though the productivity depended on the amount of air supplied. However, no enhancement of production was observed when a combination of air and chito-heptaose was provided to the cell suspension cultures.
为了刺激东北红豆杉矮紫杉细胞悬浮培养物产生紫杉醇，在F4G4培养基中使用了两种诱导子和一种生物合成前体。每种诱导子以及生物合成前体，还有两种诱导子或一种诱导子与生物合成前体的组合，都能提高紫杉醇的产量。当细胞悬浮培养在含有壳七糖（8毫克/升）和茉莉酸（21毫克/升）的F4G4培养基中进行时，产生的紫杉醇量（16.6毫克/升）最大。与对照相比，生产力提高了4.1倍。通过向培养物供应空气，紫杉醇的产量增加了，尽管生产力取决于供应的空气量。然而，当向细胞悬浮培养物提供空气和壳七糖的组合时，未观察到产量的提高。
