Hymer W C, Grindeland R E, Salada T, Cenci R, Krishnan K, Mukai C, Nagaoka S
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
J Biotechnol. 1996 Jun 27;47(2-3):289-312. doi: 10.1016/0168-1656(96)01373-9.
In this report, we describe the results of a rat pituitary cell culture experiment done on STS-65 in which the effect of cell feeding on the release of the six anterior pituitary hormones was studied. We found complex microgravity-related interactions between the frequency of cell feeding and the quantity and quality (i.e. biological activity) of some of the six hormones released in flight. Analyses of growth hormone (GH) released from cells into culture media on different mission days using gel filtration and ion exchange chromatography yielded qualitatively similar results between ground and flight samples. Lack of cell feeding resulted in extensive cell clumping in flight (but not ground) cultures. Vigorous fibroblast growth occurred in both ground and flight cultures fed 4 times. These results are interpreted within the context of autocrine and/or paracrine feedback interactions. Finally, the payload specialist successfully prepared a fresh trypsin solution in microgravity, detached the cells from their surface and reinserted them back into the culture chamber. These cells reattached and continued to release hormone in microgravity. In summary, this experiment shows that pituitary cells are microgravity sensitive and that coupled operations routinely associated with laboratory cell culture can also be accomplished in low gravity.
在本报告中，我们描述了在STS - 65上进行的大鼠垂体细胞培养实验的结果，该实验研究了细胞喂食对六种垂体前叶激素释放的影响。我们发现，细胞喂食频率与飞行中释放的六种激素中某些激素的数量和质量（即生物活性）之间存在与微重力相关的复杂相互作用。使用凝胶过滤和离子交换色谱法对不同任务日从细胞释放到培养基中的生长激素（GH）进行分析，地面和飞行样本的定性结果相似。在飞行（而非地面）培养中，不进行细胞喂食会导致大量细胞聚集。在喂食4次的地面和飞行培养中均出现了旺盛的成纤维细胞生长。这些结果是在自分泌和/或旁分泌反馈相互作用的背景下进行解释的。最后，有效载荷专家成功在微重力环境下制备了新鲜的胰蛋白酶溶液，将细胞从其表面分离，并将它们重新插入培养室。这些细胞重新附着并在微重力环境下继续释放激素。总之，该实验表明垂体细胞对微重力敏感，并且通常与实验室细胞培养相关的联合操作也可以在低重力环境下完成。
