来自杂交白杨北川杨的肉桂酸4-羟化酶同源基因的分离与分析

Isolation and analysis of cinnamic acid 4-hydroxylase homologous genes from a hybrid aspen, Populus kitakamiensis.

作者信息

Kawai S, Mori A, Shiokawa T, Kajita S, Katayama Y, Morohoshi N

机构信息

Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Oct;60(10):1586-97. doi: 10.1271/bbb.60.1586.

DOI:10.1271/bbb.60.1586

PMID:8987656

Abstract

Cinnamic acid 4-hydroxylase (CA4H) is the second enzyme involved in phenylpropanoid biosynthesis and is a member of the cytochrome P-450 superfamily. Three CA4H homologous genes, cyp73A, cyp73b, and cyp73c, and a cDNA clone of cyp73a were isolated from a genomic library and a cDNA library of a hybrid aspen; Populus kitakamiensis, and were characterized. They might be interrupted by two introns each. cyp73a and cyp73b were very similar to each other not only in coding regions but also in non-coding regions. Southern blot analysis showed that four homologous genes for CA4H constructed a small gene family in the diploid genome of P. kitakamiensis. In the promoter regions, there were many common cis-element-like sequences in phenylpropanoid biosynthesis genes.

摘要

肉桂酸4-羟化酶（CA4H）是参与苯丙烷生物合成的第二种酶，属于细胞色素P-450超家族成员。从杂种山杨（北日本杨；Populus kitakamiensis）的基因组文库和cDNA文库中分离出三个CA4H同源基因cyp73A、cyp73b和cyp73c，以及cyp73a的一个cDNA克隆，并对其进行了表征。它们可能各自被两个内含子中断。cyp73a和cyp73b不仅在编码区而且在非编码区都非常相似。Southern印迹分析表明，CA4H的四个同源基因在北日本杨的二倍体基因组中构成了一个小基因家族。在启动子区域，苯丙烷生物合成基因中有许多常见的顺式元件样序列。