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通过聚合酶链反应鉴定弓形杆菌分离株。

Identification of Arcobacter isolates by PCR.

作者信息

Harmon K M, Wesley I V

机构信息

Enteric Diseases and Food Safety Research, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA, USA.

出版信息

Lett Appl Microbiol. 1996 Oct;23(4):241-4. doi: 10.1111/j.1472-765x.1996.tb00074.x.

DOI:10.1111/j.1472-765x.1996.tb00074.x
PMID:8987697
Abstract

A polymerase chain reaction (PCR) assay was developed for the identification of the three species of Arcobacter which have been recovered from clinically ill or healthy humans and/or livestock, namely Arcobacter butzleri, Arcobacter skirrowii and Arcobacter cryaerophilus. The assay utilizes primers targeted to the genes encoding 16S rRNA of Arcobacter spp. The assay reduces the amount of time required to positively identify strains of Arcobacter.

摘要

已开发出一种聚合酶链反应(PCR)检测方法,用于鉴定从临床患病或健康的人类和/或家畜中分离出的三种弓形杆菌,即布氏弓形杆菌、斯氏弓形杆菌和嗜低温弓形杆菌。该检测方法使用针对弓形杆菌属编码16S rRNA的基因的引物。该检测方法减少了阳性鉴定弓形杆菌菌株所需的时间。

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