Hatanaka T, Hashimoto T, Kawahara T, Takami M, Asahi N, Wada R
Kuraray Co., Ltd. Okayama, Japan.
Biosci Biotechnol Biochem. 1996 Nov;60(11):1861-3. doi: 10.1271/bbb.60.1861.
Poly(vinyl alcohol) dehydrogenase (PVADH) purified from Pseudomonas sp. 113P3 catalyzed an oxidation of poly(vinyl alcohol) (PVA) in the presence of pyrroloquinoline quinone (PQQ) to give a beta-diketone structure on PVA. Although PVADH oxidized not only enzymatically oxidized PVA but also chemically oxidized PVA, PVA-degrading microorganisms, Pseudomonas sp. 113P3 and Arthrobacter tumescens sp. 52-1 grew on the enzymatically oxidized PVA, but not on the chemically oxidized PVA. This suggests that the growth of PVA-degrading microorganisms is affected by the structure of oxidized PVA.
从假单胞菌属113P3中纯化得到的聚乙烯醇脱氢酶（PVADH）在吡咯喹啉醌（PQQ）存在的情况下催化聚乙烯醇（PVA）氧化，使PVA上形成β-二酮结构。尽管PVADH不仅能氧化酶促氧化的PVA，还能氧化化学氧化的PVA，但能降解PVA的微生物，即假单胞菌属113P3和肿胀节杆菌52-1，能在酶促氧化的PVA上生长，却不能在化学氧化的PVA上生长。这表明能降解PVA的微生物的生长受到氧化PVA结构的影响。
