马铃薯查尔酮合酶基因家族一个成员的克隆与特性分析
Cloning and characterization of one member of the chalcone synthase gene family from Solanum tuberosum L.
作者信息
Jeon J H, Kim H S, Choi K H, Joung Y H, Joung H, Byun S M
机构信息
Department of Biological Science, Korea Advanced Institute of Science and Technology, Taejon, Korea.
出版信息
Biosci Biotechnol Biochem. 1996 Nov;60(11):1907-10. doi: 10.1271/bbb.60.1907.
We have isolated a chalcone synthase gene 2 (ST-CHS2) from potato by rapid amplification of cDNA ends by PCR. CHS2 cDNA had high homology to tomato LET-CHS2 (98%), petunia PHCHSJ (94%), potato ST-CHS1B (92%), petunia PHCHSA (92%), and LET-CHS1 (90%) at the overall 389-amino acid level. Genomic hybridization analysis indicated that CHS genes of potato comprise a family of at least six individual members.
我们通过PCR快速扩增cDNA末端，从马铃薯中分离出一个查尔酮合酶基因2（ST-CHS2）。在389个氨基酸的总体水平上，CHS2 cDNA与番茄LET-CHS2（98%）、矮牵牛PHCHSJ（94%）、马铃薯ST-CHS1B（92%）、矮牵牛PHCHSA（92%）和LET-CHS1（90%）具有高度同源性。基因组杂交分析表明，马铃薯的CHS基因由至少六个独立成员组成一个家族。
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