Kisidayová S
Slovak Academy of Sciences, Institute of Animal Physiology, Kosice, Slovakia.
Lett Appl Microbiol. 1996 Dec;23(6):389-92. doi: 10.1111/j.1472-765x.1996.tb01343.x.
Large entodiniomorphid ciliates taken from the rumen cannot be reliably kept in vivo, and cryopreservation was investigated as an alternative approach to storage. Three species of large entodiniomorphid ciliated (Polyplastron multivesiculatum, Epidinium ecaudatum forma caudatum and Ophryoscolex caudatus forma tricoronatus) were cryopreserved by one-step freezing, with mean recoveries of 43, 51 and 80%, respectively. For Ophryoscolex caudatus forma tricoronatus two-step freezing was investigated. The mean recovery was 30%, and of tested parameters, the equilibration time and temperature of the cryoprotective additive of dimethyl sulphoxide and the length of holding phase at -40 degrees C were found to be critical for the success of the method.
从瘤胃中获取的大型内毛目纤毛虫无法在体内可靠地保存，因此研究了冷冻保存作为一种替代的储存方法。三种大型内毛目纤毛虫（多泡多膜虫、尾裸头虫尾型和三冠尾双毛虫）通过一步冷冻法进行冷冻保存，平均复苏率分别为43%、51%和80%。对于三冠尾双毛虫，研究了两步冷冻法。平均复苏率为30%，在所测试的参数中，发现二甲基亚砜冷冻保护剂的平衡时间和温度以及在-40℃下的保持阶段时长对该方法的成功至关重要。
