适合检测血小板同种抗体的方法的比较分析：14C - 5 - 羟色胺摄取抑制、14C - 5 - 羟色胺释放及微量补体结合法

A comparative analysis of methods suitable for detection of platelet alloantibodies: inhibition of 14C-serotonin uptake, 14C-serotonin release and microcomplement fixation.

作者信息

Heinrich D, Kessler C, Mueller-Eckhardt C M

出版信息

Vox Sang. 1977;32(6):325-34. doi: 10.1111/j.1423-0410.1977.tb00654.x.

DOI:10.1111/j.1423-0410.1977.tb00654.x

Abstract

Three HLA-specific antisera were comparatively analyzed by platelet microcomplement fixation and 14C-serotonin uptake and/or 14C-serotonin release studies with platelets. In general, there was a good correlation of HLA antibody specificity exhibited in all 3 test systems. Quantitative differences in the response of platelets to HLA antisera were observed and accounted for by differences in antigen density and different types of antibodies involved. Platelet autoantibodies could not be detected by means of 14C-serotonin release from platelets in plasma.

摘要

通过血小板微量补体结合试验以及对血小板进行¹⁴C - 5 -羟色胺摄取和/或¹⁴C - 5 -羟色胺释放研究，对三种HLA特异性抗血清进行了比较分析。总体而言，在所有这三种测试系统中所显示的HLA抗体特异性具有良好的相关性。观察到血小板对HLA抗血清反应的定量差异，这可由抗原密度的差异以及所涉及的不同类型抗体来解释。通过血浆中血小板的¹⁴C - 5 -羟色胺释放无法检测到血小板自身抗体。