Studies on high-density cultivation of Vero cells with biosilon solid microcarrier.

From eight kinds of microcarriers, Biosilon has been chosen as the most suitable microcarrier for high-density cultivation of African green monkey kidney (Vero) cells. Conditions affecting high-density cultivation were studied with a 500 ml modified Wheaton spinner flask. When cultivation with a 1.5 L CelliGen bioreactor at inoculum density of 4.9 x 10(6)/ml and perfusion volume increased from 0.20 L to 3.65 L gradually, the cell density could reach 2.05 x 10(7)/ml after 22 days of cultivation. When Vesicular Stomatitis Virus (VSV) was inoculated into the cultures of 1.21 x 10(6) ml at the some MOI (multiplicity of infection), the virus titers reached 7 log TCID50/ml and 8 log TCID50/ml, respectively.