Toriniwa Hiroko, Komiya Tomoyoshi
Department of Research and Development, Research Center for Biologicals, The Kitasato Institute, 6-111 Arai, Kitamoto, Saitama, Japan.
Biologicals. 2007 Oct;35(4):221-6. doi: 10.1016/j.biologicals.2007.02.002. Epub 2007 Apr 2.
A novel oscillating bioreactor, BelloCell, was successfully applied for the cultivation of Vero cells using serum-free medium, and the production of Japanese encephalitis virus. The BelloCell requires no air sparging, pumping, or agitation, and thus provides a low shear environment. Owing to its simple design, BelloCell is extremely easy to handle and operate. Using this BelloCell (500 ml culture), Vero cells reached a maximum number of 2.8 x 10(9) cells and the Japanese encephalitis virus yield reached 6.91 x 10(11) PFU, versus 9.0 x 10(8) cells and 2.98 x 10(11) PFU using a spinner flask (500 ml) with microcarriers. The cell yield and virus production using BelloCell were markedly higher than with microcarrier culture. The neutralizing capacity of the Japanese encephalitis virus produced using BelloCell was equal to that using a microcarrier system. Therefore, these benefits should enable BelloCell to be adopted as a simple system for high population density cell culture and virus production.
一种新型振荡生物反应器BelloCell已成功应用于使用无血清培养基培养Vero细胞以及生产日本脑炎病毒。BelloCell无需空气鼓泡、泵送或搅拌，因此提供了低剪切环境。由于其设计简单，BelloCell极其易于操作和使用。使用这种BelloCell（500毫升培养物），Vero细胞达到了2.8×10⁹个细胞的最大数量，日本脑炎病毒产量达到6.91×10¹¹ PFU，而使用带有微载体的转瓶（500毫升）时，细胞数量为9.0×10⁸个，病毒产量为2.98×10¹¹ PFU。使用BelloCell的细胞产量和病毒产量明显高于微载体培养。使用BelloCell生产的日本脑炎病毒的中和能力与使用微载体系统时相同。因此，这些优势应使BelloCell能够作为一种用于高细胞密度培养和病毒生产的简单系统被采用。
