能够区分籼稻和粳稻品种的新型DNA探针。
Novel DNA probes capable of discriminating indica and japonica rice cultivars.
作者信息
Oba S, Hanzawa M, Anai T, Umeda M, Uchimiya H
机构信息
Faculty of Agriculture, Gifu University, Japan.
出版信息
DNA Seq. 1996;6(5):303-6. doi: 10.3109/10425179609020878.
We obtained two DNA clones, G93 (1.6kb) and G318 (1.7kb) from indica rice (Oryza sativa L.) cultivar Culture 340. Southern hybridization analysis indicated that both DNA probes hybridized with DNA of indica cultivars but not with japonica cultivars. Some javanica cultivars DNAs did not hybridize with these probes. Nucleotide sequence (400bp) of a part of G93 showed a 65.8% G + C content. These novel DNA probes can be used for the study of evolutional fingerprinting of rice cultivars.
我们从籼稻（Oryza sativa L.）品种Culture 340中获得了两个DNA克隆，G93（1.6kb）和G318（1.7kb）。Southern杂交分析表明，这两种DNA探针都能与籼稻品种的DNA杂交，但不能与粳稻品种的DNA杂交。一些爪哇稻品种的DNA不能与这些探针杂交。G93一部分的核苷酸序列（400bp）显示G + C含量为65.8%。这些新型DNA探针可用于水稻品种进化指纹图谱的研究。
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