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鸡铜锌超氧化物歧化酶基因的特性分析

Characterisation of the chicken Cu,Zn superoxide dismutase gene.

作者信息

Stanton J L, Wilton S D, Laing N G

机构信息

Australian Neuromuscular Research Institute, Queen Elizabeth II Medical Centre, Nedlands, Australia.

出版信息

DNA Seq. 1996;6(6):357-60. doi: 10.3109/10425179609047575.

DOI:10.3109/10425179609047575
PMID:8988375
Abstract

A PCR product was generated from embryonic chicken spinal cord cDNA using primers designed to conserved regions of the human and bovine amino and carboxyl-terminal coding sequences of the Cu,Zn superoxide dismutase (SOD1, EC 1.15.1.1) gene. DNA sequencing confirmed this product to be the chicken homologue of the SOD1 gene. This sequence was compared to SOD1 from bovine, human and Xenopus laevis. Important structural features of SOD1 are shown to be conserved in the chicken gene.

摘要

使用针对人及牛铜锌超氧化物歧化酶(SOD1,EC 1.15.1.1)基因的氨基和羧基末端编码序列保守区域设计的引物,从鸡胚胎脊髓cDNA中生成了一个PCR产物。DNA测序证实该产物是SOD1基因的鸡同源物。将该序列与牛、人和非洲爪蟾的SOD1进行了比较。结果表明,SOD1的重要结构特征在鸡基因中是保守的。

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