Blasco Casares F J, Saladié Roig J M, Encabo Mayoral B
Department of Urology, Hospital Municipal de Badalona, Spain.
Arch Esp Urol. 1996 Nov;49(9):995-1002.
The ureteral changes that occur when rat ureter is used as an autologous free graft were evaluated using histomorphometrical methods.
The normal values were determined in ureters from normal rats (NU group) and compared with those of a control group undergoing ureterolysis, as well as with two groups in which the ureter was used as an autologous free graft (in one of these groups the ureter was integrated into the urinary tract by anastomosis, whereas in the other group the ureter was not integrated). The main histomorphometrical parameters were thickness or length and surface or area. Thickness measurements were made using a micrometer. For area determinations, paper prints of light micrographs from each segment were measured using a Koizumi KP-90 digital planimeter. A non-parametric variance analysis (Kruskal-Wallis test) was used to compare the results. These results were compared with those of the Wilcoxon test. The results are expressed as different levels of alpha, (0.05, 0.01 and 0.001).
When p < 0.01, there were practically no statistically different variables between NU and AU. Comparison of NU to SC and SC to AU showed only seven statistically different variables. Muscle layer thickness was different only when the comparison was made with the RUb group. When p < 0.001, RUa and RUb were not different, nor were NU and AU or SC and NU. Differences were observed between RUb and all the other groups. RUa was different from NU, SC, or AU in 3, 4 or 5 of the 18 variables analyzed. Most of the surface variables were significantly different in RUb in comparison to the other groups. There were also differences between RUa and NU, as well as between NU and SC, in the area of the lamina propria.
Initial ischemia and denervation are not the cause of the observed changes, as these changes are different in both types of free graft. Histomorphometrical studies constitute a useful method in the assessment of differences between ureteral free graft groups. None of the histomorphometric findings is inconsistent with the use of the rat ureter as a free graft.
采用组织形态计量学方法评估将大鼠输尿管用作自体游离移植物时发生的输尿管变化。
测定正常大鼠输尿管（NU组）的正常值，并与输尿管松解术对照组以及输尿管用作自体游离移植物的两组（其中一组输尿管通过吻合术与尿路整合，而另一组输尿管未整合）进行比较。主要组织形态计量学参数为厚度或长度以及表面积或面积。厚度测量使用测微计。对于面积测定，使用小泉KP - 90数字平面仪测量每个节段光镜照片的纸质打印件。采用非参数方差分析（Kruskal - Wallis检验）比较结果。将这些结果与Wilcoxon检验结果进行比较。结果以不同水平的α（0.05、0.01和0.001）表示。
当p < 0.01时，NU组和AU组之间实际上没有统计学上的差异变量。NU组与SC组以及SC组与AU组的比较仅显示出7个统计学上的差异变量。仅在与RUb组进行比较时，肌层厚度存在差异。当p < 0.001时，RUa组和RUb组之间没有差异，NU组和AU组之间以及SC组和NU组之间也没有差异。在RUb组与所有其他组之间观察到差异。在分析的18个变量中，RUa组在3、4或5个变量上与NU组、SC组或AU组不同。与其他组相比，RUb组的大多数表面变量存在显著差异。在固有层面积方面，RUa组与NU组之间以及NU组与SC组之间也存在差异。
最初的缺血和去神经支配不是观察到的变化的原因，因为在两种类型的游离移植物中这些变化是不同的。组织形态计量学研究是评估输尿管游离移植物组之间差异的一种有用方法。没有任何组织形态计量学结果与将大鼠输尿管用作游离移植物相矛盾。
