Dekhtiareva N I, Medvedeva T E, Poliakov Iu M, Golubev D B, Aleksandrova G I
Vopr Virusol. 1977 Mar-Apr(2):173-7.
A high sensitivity of influenza virus to replication at a high temperature and a reduction of the temperature optimum of the neuraminidase activity are important genetic markers correlating with the degree of attenuation for the man. Cold-adapted variants of influenza virus produced in the course of passages at a low (25-28 degrees C) temperature are thermo-sensitive. The passage of the viruses at the optimal temperature (32 degrees C) does not change the range of thermal sensitivity even in variants, harmless for adult persons. The interfering activity of influenza viruses with different levels of human virulence was studied. A considerable portion of the examined cold-adapted strains differs from the original virulent strains in the capacity to interfere with the challenge virus in chick embryo fibroblast cell culture. However some thermosensitive variants as well as original strains do not react in interference tests. This indicates the lack of complete correlation between the interfering activity, thermal sensitivity and human virulence of influenza virus.
流感病毒在高温下对复制具有高敏感性以及神经氨酸酶活性的最适温度降低，是与人的减毒程度相关的重要遗传标记。在低温（25 - 28摄氏度）传代过程中产生的流感病毒冷适应变种对温度敏感。即使对于对成人无害的变种，病毒在最适温度（32摄氏度）下传代也不会改变热敏感范围。研究了具有不同人类毒力水平的流感病毒的干扰活性。在鸡胚成纤维细胞培养中，相当一部分检测的冷适应毒株在干扰攻击病毒的能力上与原始毒株不同。然而，一些热敏感变种以及原始毒株在干扰试验中没有反应。这表明流感病毒的干扰活性、热敏感性和人类毒力之间缺乏完全相关性。
