Holder I A, Haidaris C G
Can J Microbiol. 1979 May;25(5):593-9. doi: 10.1139/m79-085.
The effects on mortality of supplemental injections of protease and elastase were determined in burned mice infected with non-lethal inocula of a toxin-producing but non-proteolytic-enzyme-producing strain of Pseudomonas aeruginosa. When a variety of solutions containing proteolytic enzyme were injected under these conditions, the mortality increased significantly. This did not occur when organisms other than P. aeruginosa were used. Injections of the enzyme solutions alone were non-lethal. Injection of a solution of alpha 2-macroglobulin, which was shown to inhibit proteolytic activity, together with a proteolytic enzyme--toxin producing strain of P. aeruginosa caused a significant delay in mortality when compared with controls. It was concluded that protease, elastase, and toxin production were necessary for P. aeruginosa to express full virulence in the burned mouse model.
在感染了产毒素但不产生蛋白水解酶的铜绿假单胞菌非致死接种物的烧伤小鼠中，测定了补充注射蛋白酶和弹性蛋白酶对死亡率的影响。在这些条件下注射含有蛋白水解酶的各种溶液时，死亡率显著增加。当使用铜绿假单胞菌以外的微生物时，这种情况不会发生。单独注射酶溶液不会致死。与对照组相比，注射显示能抑制蛋白水解活性的α2-巨球蛋白溶液以及蛋白水解酶-产毒素铜绿假单胞菌菌株会导致死亡率显著延迟。得出的结论是，蛋白酶、弹性蛋白酶和毒素产生对于铜绿假单胞菌在烧伤小鼠模型中表达完全毒力是必要的。
